Vitamin E Ameliorates Sodium Fluoride-Induced Morphometric, Histomorphological, and Biochemical Changes on the Kidney of Adult Wistar Rat

Jerry Akasi, David; Aniekan Imo, Peter; Oyono, Igiri Anozeng

doi:10.61882/rabms.11.3.295

Volume 11, Issue 3 (8-2025) Journal of Research in Applied and Basic Medical Sciences 2025, 11(3): 295-304 | Back to browse issues page

‎ 10.61882/rabms.11.3.295

Ethics code: 15/PG/BMS/AN/003

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Jerry Akasi D, Aniekan Imo P, Oyono I A. Vitamin E Ameliorates Sodium Fluoride-Induced Morphometric, Histomorphological, and Biochemical Changes on the Kidney of Adult Wistar Rat. Journal of Research in Applied and Basic Medical Sciences 2025; 11 (3) :295-304
URL: http://ijrabms.umsu.ac.ir/article-1-423-en.html

Vitamin E Ameliorates Sodium Fluoride-Induced Morphometric, Histomorphological, and Biochemical Changes on the Kidney of Adult Wistar Rat

David Jerry Akasi ^*

, Peter Aniekan Imo

, Igiri Anozeng Oyono

Department of Anatomy, Faculty of Basic Medical Science, University of Nigeria, Nsukka Nasarawa State College of Nursing Sciences, Lafia, Nigeria , jerry.david.pg90800@unn.edu.ng

Abstract: (113 Views)

Background Sodium fluoride, NaF, usage in dental products and drinking water is one of the most promising methods of dental caries management; however, its toxicity generates free radicals. Vitamin E is a recognized antioxidant that helps protect against tissue damage. The study was aimed at investigating whether vitamin E at the tolerable upper intake level could ameliorate NaF-induced toxicity on kidney morphometry, histomorphology, and serum biochemical markers of renal function in adult male Wistar rats.
Methods A total of 30 male Wistar rats weighing 130-180 g were randomly divided into six groups of five animals each. Group A and B served as the control and received 1 ml of distilled water and 1 ml of Tween 80®; Group C received 5 mg/kg body weight of NaF, Group D received 20 mg/kg body weight of NaF while Group E and F received 5 mg/kg and 20 mg/kg and 14.3 mg/day of vitamin E orally, respectively.
Results After 45 days, morphometry of the kidney showed a significant (p < 0.05) increase in length, thickness, and width of the inferior pole in NaF groups only, but significantly decreased in Group E compared with the control. Histomorphology showed various changes in Groups C and D, while Groups E and F showed mild modifications compared to the control. Masson’s trichrome stain showed pale-stained collagen in NaF groups (C and D) but increased staining in vitamin E groups (E and F). Serum creatinine and urea levels were significantly increased in Group D but decreased in Groups E and F. Electrolytes (Na+, Cl−, and HCO3−) were significantly increased in the NaF-treated groups but decreased with vitamin E compared to the control.
Conclusion NaF induced significant changes in kidney morphometry, histomorphology, and serum biochemical markers of renal function. Vitamin E administration at the upper tolerable intake level can mitigate the changes.
Background Sodium fluoride, NaF, usage in dental products and drinking water is one of the most promising methods of dental caries management; however, its toxicity generates free radicals. Vitamin E is a recognized antioxidant that helps protect against tissue damage. The study was aimed at investigating whether vitamin E at the tolerable upper intake level could ameliorate NaF-induced toxicity on kidney morphometry, histomorphology, and serum biochemical markers of renal function in adult male Wistar rats.
Methods A total of 30 male Wistar rats weighing 130-180 g were randomly divided into six groups of five animals each. Group A and B served as the control and received 1 ml of distilled water and 1 ml of Tween 80®; Group C received 5 mg/kg body weight of NaF, Group D received 20 mg/kg body weight of NaF while Group E and F received 5 mg/kg and 20 mg/kg and 14.3 mg/day of vitamin E orally, respectively.
Results After 45 days, morphometry of the kidney showed a significant (p < 0.05) increase in length, thickness, and width of the inferior pole in NaF groups only, but significantly decreased in Group E compared with the control. Histomorphology showed various changes in Groups C and D, while Groups E and F showed mild modifications compared to the control. Masson’s trichrome stain showed pale-stained collagen in NaF groups (C and D) but increased staining in vitamin E groups (E and F). Serum creatinine and urea levels were significantly increased in Group D but decreased in Groups E and F. Electrolytes (Na+, Cl−, and HCO3−) were significantly increased in the NaF-treated groups but decreased with vitamin E compared to the control.
Conclusion NaF induced significant changes in kidney morphometry, histomorphology, and serum biochemical markers of renal function. Vitamin E administration at the upper tolerable intake level can mitigate the changes.

Keywords: Histomorphology, Kidney, Morphometry, Renal function, Sodium fluoride, Vitamin E

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Type of Study: orginal article | Subject: Nephrology

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