en Evaluation of stemness genes expression of OCT4, SOX2, Nanog, C-Myc and surface marker of CD133 on myeloma cells تخصصي Special پژوهشي orginal article Cancer stem cells that have the ability to proliferation and self-renewal were cause to drug resistance and metastasis in malignancies. In multiple myeloma, these cancer stem cells (MMCSCs) play a very important role in the recurrence of the disease. Detection of these cells can create new ways for treating these patients. In this study, stemness genes and phenotypic markers were investigated as the methods for identifying cancer stem cells in multiple myeloma.Expression of stemness genes of OCT4, SOX2, Nanog, C-Myc and target genes of STAT3 and TCF3 by PCR and Real Time PCR and also expression of surface markers of CD19, CD33, CD133, CD38 and CD56 by flow cytometry in RPMI8226 and U266 were studied<span dir="RTL">.</span>The results of the study showed that myeloma cells were express the stemness genes, and also CD133 surface marker was express in the some of myeloma cells. Identification of myeloma cancer stem cells can be crucial in the diagnosis, treatment and prognosis of multiple myeloma patients. CD133+ myeloma cells that express stemness genes are Multiple Myeloma Cancer Stem Cells (MMCSC) that can be considered as therapeutic targets. Stemness genes, Myeloma cell, Immunophenotyping markers, MMCSC, CD133 96 103 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-101-16&slc_lang=en&sid=1 Ramin Saadatian kharajo saadatian@umsu.ac.ir 1003194753284600772 1003194753284600772 No Department of hematology, Faculty of medical sciences, Tarbiat Modares University, Tehran, Iran Saeid Abroun Email: abroun@modares.ac.ir 1003194753284600773 1003194753284600773 Yes Department of hematology, Faculty of medical sciences, Tarbiat Modares University, Tehran, Iran Masoud Soleimani soleim_m@modares.ac.ir 1003194753284600774 1003194753284600774 No Department of hematology, Faculty of medical sciences, Tarbiat Modares University, Tehran, Iran