Journal of Research in Applied and Basic Medical Sciences
Journal of Research in Applied and Basic Medical Sciences
Medical Sciences
http://ijrabms.umsu.ac.ir
1
admin
2717-0098
8
10.61186/rabms
14
8888
13
en
jalali
1399
3
1
gregorian
2020
6
1
6
3
online
1
fulltext
en
Characterization of ERG11 Gene in Drug-Resistant Candida Albicans Isolated from Iranian Cases of Recurrent Vulvovaginal Candidiasis
Characterization of ERG11 Gene in Drug-Resistant Candida Albicans Isolated from Iranian Cases of Recurrent Vulvovaginal Candidiasis
تخصصي
Special
پژوهشي
orginal article
<strong><em>Background & Aims</em></strong>: <em>Candida albicans</em> is the most common fungal pathogen of human infections. <em>C. albicans</em> is responsible for significant mucosal infections such as vulvovaginitis in women. Azoles inhibit the cytochrome P<sub>450</sub> 14<em>α</em>-lanosterol demethylase, as a part of the ergosterol biosynthetic pathway is encoded by the <em>ERG11</em> gene. Some mutations in <em>ERG 11</em> could cause resistance to azole drugs. Detection of the mutations of the gene in the present study helped us to explain drug resistances in some vaginal isolates of <em>C. albicans</em> and other Candida species.<br>
<strong><em>Materials & Methods:</em></strong> A multicenter, experimental study was conducted at Cellular and Molecular Research Center and Kowsar Gynecology Center affiliated to UMSU from October 2016 to July 2017. Women with symptomatic vaginitis (20-45 years old) were asked to take part in the study. 192 women allowed vaginal swabs to be obtained. For the identifications, culture on SGA4% and CHROM agar Candida were conducted followed by PCR-RFLP. A disc diffusion method was performed based on the standard guideline of the National Committee for Clinical Laboratory Standards (NCCLS) to determine level of susceptibility against fluconazole and clotrimazole (most current use for the treatment of VVC). DNA extraction and PCR amplification of the <em>ERG11</em> gene were performed.<br>
<strong><em>Results</em></strong><strong>:</strong> As we showed in the Table (1), 69.1% of all <em>Candida</em> isolates carried the <em>ERG11 </em>gene. It was detected in 49(68.1%), 5 (55.6%), and 7(77.8%) cases of <em>C. albicans, C. krusei,</em> and <em>C. glabrata,</em> respectively. Among the <em>C. albicans</em> isolates resistant to Clotrimazole, 8(53.3%) had <em>ERG11</em> gene while 7(46.7%) did not. Among all the <em>C. glabrata</em> isolates resistant to Clotrimazole, 40% carried <em>ERG11</em> while 60% did not show the gene. Also, ERG11 gene was detected in 50% of the isolated <em>C. glabrata</em>. <em>ERG11</em> gene was observed in 53.3% of <em>C.krusei</em> isolates resistant to Clotrimazole and 52% of those of resistant to Fluconazole.<br>
<strong><em>Conclusion</em></strong><strong>:</strong> As an approximate finding, Azole resistance in the present study could be attributed to mutations in ERG11 gene
ERG11 Gene, Candida Albicans, Vulvovaginal Candidiasis
153
159
http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-101-19&slc_lang=en&sid=1
Kambiz
Diba
diba.k@umsu.ac.ir
1003194753284600867
1003194753284600867
Yes
Associate Professor in Medical Mycology, Molecular and Cellular Research Center, Urmia University of Medical Sciences, Urmia, Iran
Shahram
Khademvatan
khademvatan@yahoo.com
1003194753284600868
1003194753284600868
No
Professor in Medical Parasitology, Molecular and Cellular Research Center, Urmia University of Medical Sciences, Urmia, Iran
Kowsar
Jaafari
monavvar03@gmail.com
1003194753284600869
1003194753284600869
No
Medical Mycologist, Esfahan University of Medical Sciences
Soudabe
Abharian
monavvar03@gmail.com
1003194753284600870
1003194753284600870
No
Medical Microbiologist, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran