Journal of Research in Applied and Basic Medical Sciences
Journal of Research in Applied and Basic Medical Sciences
Medical Sciences
http://ijrabms.umsu.ac.ir
1
admin
2717-0098
8
10.61186/rabms
14
8888
13
en
jalali
1400
9
1
gregorian
2021
12
1
7
3
online
1
fulltext
en
بررسی فعالیت های ضد دیابتیکی و آنتی اکسیدانی عصاره برگ های سیلیندریکوم ENTANDROPHRAGMA در موش های صحرایی نر ویستار
Antidiabetic and Antioxidant Activities of Extract of Entandrophragma Cylindricum (Sprague) Leaves in Male Wistar Rats
عمومى
General
پژوهشي
orginal article
<strong><em>Background & Aims</em></strong>: <em>Entandrophragma cylindricum</em> (<em>EC) is</em> a tree with a widespread presence in various West African countries. It has wide folkloric use as an anti-sickling, antimalarial, analgesic, anti-inflammatory, and is widely used traditionally in treating diabetes across West Africa. The purpose of this research is to evaluate the antidiabetic potential of the methanolic leaf extract of <em>Entandrophragma cylindricum</em> (<em>EC) </em>in rats.<br>
<strong><em>Materials and Methods</em></strong><em>:</em> Induction of Diabetes mellitus was done by Streptozotocin (STZ) via intraperitoneal route injection. Animals were apportioned into five groups (n=5) for contrasting the activity of <em>EC</em> at three <em>EC</em> doses (25 mg/kg, 50 mg/kg, 150 mg/kg p.o.) against the standard drug (Glibenclamide) and control groups. Rats having elevated glucose levels above 250 mg/dL were considered diabetic and used for the study. Normoglycemic test, Oral Glucose Tolerance Test (OGTT), STZ-Induced diabetes, in-vitro antioxidant properties of <em>EC</em> extract, and <em>in</em> vivo antioxidant property of the serum were assessed.<br>
<strong><em>Results</em></strong>: Phytochemistry revealed the presence of tannins, flavonoids, saponins, alkaloids, terpenoids, deoxy-sugars, and anthraquinones. The three doses of <em>EC</em> (150, 50, and 25 mg/kg) used in the study caused a significant decrease in blood glucose levels in the STZ-induced diabetic rat model. Also, <em>EC</em> (150, 50, and 25 mg/kg) produced a significant (p< 0.001) increase respectively in the body weight from day 0 to day 30 when compared with the untreated diabetic rat. Our results indicated that <em>EC</em> might be a potent free radical scavenger, as it scavenged the 2,2 -diphenyl picrylhydrazyl (DPPH) radical, hydroxyl radical, and NO radicals <em>in</em> vitro. <em>EC</em> also showed a significant anti-lipid peroxidative effect <em>in</em> vivo. Histologic analysis revealed the regenerative impact of EC on the β-cells of diabetic rats.<br>
<strong><em>Conclusion</em></strong><em>:</em> Findings revealed that <em>EC</em> possess antihyperglycemic and antioxidant effects.
Entandrophragma cylindricum, Diabetes mellitus, antioxidant, antidiabetic
108
121
http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-160-1&slc_lang=en&sid=1
Farouk
Oladoja
adedeji.oladoja@oouagoiwoye.edu.ng
10031947532846001223
10031947532846001223
Yes
Olabisi Onabanjo University
Emmanuel
Irokosu
emmanuel.irokosu@oouagoiwoye.edu.ng
10031947532846001224
10031947532846001224
No
Olabisi Onabanjo University
Oluwafemi
Kale
Kale.oluwafemi@oouagoiwoye.edu.ng
10031947532846001225
10031947532846001225
No
Olabisi Onabanjo University
Taiwo
Olubodun-Obadun
olubodunobaduntaiwo@gmail.com
10031947532846001226
10031947532846001226
No
University of Lagos