en تخصصي Special پژوهشي orginal article <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span lang="EN-IN" style="font-size:9.0pt">Non-Fermenting Gram-Negative Bacilli [NFGNB] which are considered as environmental contaminants have emerged as multidrug-resistant bacteria and are of serious concern to the treating physician. The aim of this study was to determine the proportion of metallo-beta-lactamase producing <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter </i>species in clinical samples received in the Microbiology Laboratory and to study the antibiotic sensitivity pattern of <a name="_Hlk157848649">metallo-beta-lactamase </a>(MBL) producing strains</span><span style="font-size:9.0pt">.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Materials & Methods:</span></i></b> &nbsp;<span lang="EN-IN" style="font-size:9.0pt">Various samples (pus, sputum, urine, blood and body fluids, etc.) were processed according to standard protocols. <i>P.aeruginosa and Acinetobacter spp</i> were isolated and identified with the help of various culture media, staining methods and biochemical reactions. Antibiotic susceptibility test was done by using Kirby-bauer disc diffusion method. MBL producers were identified using CLSI guidelines.</span><b><span style="font-size:9.0pt"></span></b></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Results</span></i></b><b><span style="font-size:9.0pt">:</span></b> &nbsp;<span lang="EN-IN" style="font-size:9.0pt">Out of 600 positive culture isolates from various samples, 65 (10.8%) were non-fermenting gram-negative bacilli, 40 (61.53%) were <i>Pseudomonas aeruginosa</i>, and 25 (38.47%) were <i>Acinetobacter</i> species. The overall incidence of MBL positive isolates in our study was 12.3% (8 of 65). All the isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter</i>species were 100% sensitive to Colistin and Tigecycline.</span><b><span style="font-size:9.0pt"></span></b></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><span style="font-size:9.0pt">: &nbsp;</span><span lang="EN-IN" style="font-size:9.0pt">The study helps in understanding the antibiotic resistance pattern of isolates causing nosocomial infections, helping clinicians in making appropriate antibiotic choices as an empirical therapy and the policy-makers to bring out the measures in controlling the superbugs.</span><span style="font-size:9.0pt"></span></span></span></span><br> &nbsp; Acinetobacter Species, Multidrug Resistant, Metallo-Beta-Lactamase, Non-Fermenting Gram-Negative Bacilli, Pseudomonas Aeruginosa, Superbugs 72 79 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-249-1&slc_lang=en&sid=1 Archana Rao K archanaswaroop79@gmail.com 10031947532846002484 10031947532846002484 Yes Post-graduation first year, Department of Dermatology, JSS Medical college, India Aradita C archana.vasista@gmail.com 10031947532846002485 10031947532846002485 No Assistant professor, Department of microbiology, Rajarajeswari medical college and hospital. Bengaluru, India Lakshminarayana SA lakshmi.kims@gmail.com 10031947532846002486 10031947532846002486 No Professor and Head, Department of microbiology, Rajarajeswari medical college and hospital. Bengaluru, India