en Parasitology Parasitology پژوهشي orginal article <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span style="font-size:9.0pt">Malaria disease imposes a substantial global health burden, urging the search for effective treatments amid escalating drug resistance. This study investigates the antiplasmodial potential of Azanza garckeana root bark extract in Plasmodium berghei-infected mice and evaluates its impact on hematological indices.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Materials & Methods:</span></i></b><span style="font-size:9.0pt"> In this experimental research, we studied on six groups of 30 mice (Groups A to F) comprising of five mice per group. Group A was only given food and water with no inoculation and treatment, B was inoculated with Plasmodium berghei but no treatment was given, C was infected and treated with artemether while D E F were infected and treated with 100mg/kg, 200mg/kg and 300mg/kg of Azanza garckeana root-bark extract respectively for 4 days. Parasitemia levels, chemo-suppressive effect and hematological parameters were assessed on four different days following the start of the treatment. The findings were expressed as mean &plusmn; SEM. One-way analysis of variance was used to find the differences between the four groups, and p < 0.05 was considered statistically significant.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Results:</span></i></b><span style="font-size:9.0pt"> Phytochemical analysis revealed diverse bioactive compounds in the extract. The 4-day suppressive test demonstrated substantial antiplasmodial activity, with the 300 mg/kg dose achieving an 88.17% chemo-suppressive effect, comparable to Artemether&#39;s efficacy. Significant (p < 0.05) increases in hemoglobin, packed cell volume, red blood cells, white blood cells, and platelets were seen in hematological examinations in intervention groups, especially with the 300 mg/kg dosage.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Conclusions:</span></i></b><span style="font-size:9.0pt"> Azanza garckeana root-bark extract exhibited potent antiplasmodial effects, possibly mediated by identified phytochemicals. The dose-dependent chemosuppression and modulation of hematological parameters underscore its potential as an alternative antimalarial therapy in mammals.</span></span></span></span><br> <span style="font-size:12pt"><span style="background:#d9d9d9"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.0pt"></span></span></span></span></span> Antiplasmodial Activity, Azanza Garckeana, Hematological Indices, Plasmodium Berghei 213 223 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-369-1&slc_lang=en&sid=1 Mahmoud Suleiman Jada jadasm84@gmail.com 10031947532846003129 0009-0006-5943-2328 Yes Department of Biochemistry, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa state, Nigeria Yusuf Umar yusufumarkombani@gmail.com 10031947532846003130 0009-0004-7258-2940 No Department of Biochemistry, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa state, Nigeria Abdullahi Usman Wurochekke wchekke@gmail.com 10031947532846003131 0009-0009-8801-3212 No Department of Biochemistry, Faculty of Life Sciences, Modibbo Adama University, Yola, Adamawa state, Nigeria