en Other Other پژوهشي orginal article <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span style="font-size:9.0pt">Doxorubicin is a widely used antineoplastic agent for the treatment of solid tumors but its use is limited by its several severe tissue and organ toxicities. This study investigated changes in liver and spleen as a result of toxicity produced by Doxorubicin and the protective role of aqueous leaf extract of <i>J. Tanjorensis</i>.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Materials & Methods</span></i></b><b><span style="font-size:9.0pt">:</span></b> <span style="font-size:9.0pt">In this experimental study, rats were divided into 5 groups as follows: </span><span lang="EN-GB" style="font-size:9.0pt">Group 1 served as control and orally received normal saline once daily. Doxorubicin (15 mg/kg) was administered to Group 2&nbsp; from day 10. Group 3 received <i>J. Tanjorensis</i> (300 mg/kg, orally) once daily for 12 days. Group 4 received <i>J. Tanjorensis</i> (300 mg/kg, orally) once daily for 12 days and Doxorubicin (15 mg/kg) from day 10. Group 5 received Vitamin C (100 mg/kg, orally) once daily for 12 days, and Doxorubicin (15 mg/kg) from day 10. Doxorubicin administration was done intraperitoneally for three consecutive days. </span><span style="font-size:9.0pt">Sera samples were collected and used to assess liver function enzymes and synthetic molecules. Liver and spleen tissues were used to examine histopathological analysis. Data were analyzed by SPSS v.20 at a significance level of <i>P<0.05</i>. </span>&nbsp;<b><span style="font-size:9.0pt"></span></b></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Results</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> &nbsp;Administration of Doxorubicin caused significant increase in Alanine Transaminase (ALT), Aspartate Transaminase (AST), Acid Phosphatase (ACP), and total bilirubin (P values below 0.05), and a significant decrease in total protein and albumin compared to the control and <i>J. Tanjorensis </i>administered rats (P values below 0.05). </span><span lang="EN-GB" style="font-size:9.0pt">The histopathological evaluation of liver tissue in the Doxorubicin injected rats revealed congestion, hemorrhagic necrosis</span><span style="font-size:9.0pt">, sinusoidal dilation, and mononuclear cell infiltration</span><span lang="EN-GB" style="font-size:9.0pt">. Similarly, histology of spleen tissue in Doxorubicin administered rats showed </span><span style="font-size:9.0pt">degeneration and congestion, disintegrated peri-arteriolar lymphoid sheath, granuloma formation, and</span><span lang="EN-GB" style="font-size:9.0pt"> necrosis of lymphoid follicles. However, liver and spleen of rats given Doxorubicin and <i>J. Tanjorensis</i></span><span style="font-size:9.0pt"> showed reversal of liver function enzymes and synthetic ability towards normalcy, reduced signs of damage as well as recovering peri-arteriolar lymphoid sheath.</span><b><span style="font-size:9.0pt"></span></b></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span style="font-size:9.0pt">Our study found that <i>J. Tanjorensis</i> is effective in preventing liver and spleen damage caused by Doxorubicin.</span></span></span></span><span style="font-size:12pt"><span style="background:#d9d9d9"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.0pt"></span></span></span></span></span> Doxorubicin, Jatropha Tanjorensis, Liver, Rat, Spleen, Toxicity 256 265 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-224-3&slc_lang=en&sid=1 Usunomena Usunobun usunobun.usunomena@edouniversity.edu.ng 10031947532846003196 10031947532846003196 Yes Department of Biochemistry, Faculty of Basic Medical Sciences, Edo State University Uzairue, Edo State, Nigeria Onotse Agunu onotse26@gmail.com 10031947532846003197 10031947532846003197 No Department of Biochemistry, Faculty of Basic Medical Sciences, Edo State University Uzairue, Edo State, Nigeria Benjamin Okechukwu benokeson@gmail.com 10031947532846003198 10031947532846003198 No Department of Biochemistry, Faculty of Natural and Applied Sciences, State University of Medical and Applied Sciences, Igbo-Eno, Nsukka, Enugu State, Nigeria