en Microbiology Microbiology پژوهشي orginal article <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<i><span style="font-size:9.0pt">Citrobacter</span></i><span style="font-size:9.0pt">, a member of the Enterobacteriaceae family known to be a normal intestinal flora and an opportunistic pathogen is now increasingly found to cause a variety of infections in community as well as hospital settings. It was initially considered a low virulence pathogen but is now found to cause multi-drug resistant infections with high morbidity and mortality.<b> </b>To determine the prevalence of infections caused by Citrobacter spp. and theirAntibiotic sensitivity pattern.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Material & Methods</span></i></b><b><span style="font-size:9.0pt">: </span></b><span style="font-size:9.0pt">A Laboratory records-based Cross-sectional study was undertaken retrospectively wherein the laboratory data about 50 <i>Citrobacter</i> spp. isolates obtained from 1628 clinical samples processed over 18 months were retrieved and analyzed at Department of Microbiology, Government Medical College-Datia, Central India in January 2024.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Result</span></i></b><b><span style="font-size:9.0pt">: </span></b><span style="font-size:9.0pt">Out of the 1628 samples processed, significant bacterial growth was reported in 770 samples out of which 50 were found to be positive for <i>Citrobacter</i> spp. (6.4%). Isolation rate for <i>Citrobacter</i> spp. was reported to be 3.1 %.The majority of isolates were obtained from Urine (49%) and pus (45%) samples with <i>C. koseri </i>being dominant in urine and <i>C. freundii </i>in pus. Amongst these isolates, 52% were <i>C.koseri</i>, 46% were <i>C.freundii</i> and 4% were other species.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><b><span style="font-size:9.0pt">: </span></b><span style="font-size:9.0pt">The findings of the study conducted in January 2024 at Datia (Madhya Pradesh) indicate a change in susceptibility trends of emerging pathogens like <i>Citrobacter</i> spp. exhibiting resistance to routinely prescribed antimicrobials, stressing the need for an appropriate action plan involving periodic surveillance, antimicrobial stewardship, and strict implementation of infection control practices.</span></span></span></span><span style="font-size:12pt"><span style="background:#d9d9d9"><span style="line-height:16.0pt"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.0pt"></span></span></span></span></span> Citrobacter spp., Clinical isolates,Antimicrobial susceptibility pattern, Samples 285 290 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-410-1&slc_lang=en&sid=1 Abhishek Mehta abhishekmehta623@gmail.com 10031947532846003228 10031947532846003228 Yes Associate Professor, Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India Vismit Mungi vismit235@gmail.com 10031947532846003229 10031947532846003229 No Senior Resident, Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India Sagar Jain sagarjain1194@gmail.com 10031947532846003230 10031947532846003230 No PG student(JR-1), Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India