Journal of Research in Applied and Basic Medical Sciences

en A study on telomere length and 25-hydroxy vitamin D in association with glycaemic status in patients with diabetes mellitus Other Other پژوهشي orginal article Background & Aims: Telomere length is affected by vitamin D levels, and a relationship exists between telomere length and type 2 diabetes mellitus (T2DM), a condition associated with aging. This study aimed to investigate the connection between telomere length and 25(OH)D levels in diabetic individuals exhibiting various glycaemic profiles. Materials & Methods: Glycated hemoglobin (HbA1c) levels and vitamin D concentrations were measured in a cohort of 60 patients diagnosed with type 2 diabetes mellitus (T2DM), as well as in a control group of age-matched healthy individuals without diabetes. The analysis of telomere length was performed using quantitative real-time polymerase chain reaction (qPCR). Results: The findings revealed that the average telomere length (kb/genome) was significantly decreased in the diabetic group in comparison with the control group (diabetics: 4.21 ± 1.45 [3.29 - 4.65]; controls: 5.35 ± 1.85 [4.27-6.28]). Vitamin D levels were found to be notably lower in the diabetic cases (20.2 ± 8.5 vs. 51.52 ± 11.5, P = 0.0001). In the diabetic cohort, the Telomere length exhibited a significant negative correlation with HbA1c (rho = -0.97; P = 0.0002) and fasting blood sugar levels (rho = -0.95; P = 0.0003). Patients receiving insulin treatment demonstrated shorter telomere lengths compared to those on oral medication (4.32 ± 1.51 vs. 5.19 ± 1.71, P = 0.07). Insulin-treated individuals also had lower vitamin D levels than their orally treated counterparts (21.5 ± 9.5 vs. 50.8 ± 12.5, P = 0.004). Discussion: The findings reveal an inverse relationship between HbA1c levels and telomere length, highlighting a possible connection between glycemic control and cellular aging in diabetic individuals. Furthermore, although there is an indication that vitamin D deficiency may influence telomere shortening, the observed correlation is rather weak. Conclusion: Vitamin D deficiency, as well as higher HbA1c levels shortens the Telomere length; therefore, Vitamin D supplementation with optimized glycaemic control may contribute to preserving the telomere integrity in diabetic individuals. Aging, Glycaemic control, Telomere length, Type 2 diabetes mellitus, Vitamin D 207 218 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-473-1&slc_lang=en&sid=1 Mahaboob Vali Shaik research.nmc1@gmail.com 10031947532846004178 10031947532846004178 Yes Department of Research, Nimra Institute of Medical Sciences, Vijayawada, India. Munni Shaik munnishaik78@gmail.com 10031947532846004179 10031947532846004179 No Department of Research, Nimra Institute of Medical Sciences, Vijayawada, India. John basha Shaik research.nmch@rediffmail.com 10031947532846004180 10031947532846004180 No Department of Biochemistry, MNR Medical College & MNR University, Sangareddy Babulal Sabinkar research.nmch1@rediffmail.com 10031947532846004181 10031947532846004181 No Department of Pulmonology, Nimra Institute of Medical Sciences, Vijayawada, India. Nissi Molli shynee.pri@rediffmail.com 10031947532846004182 10031947532846004182 No International National Indian School, Al Jubail, Saudi Arabia Swarna deepak kuragayala syamakumar.n@rediffmail.com 10031947532846004183 10031947532846004183 No Department of Critical Care Medicine, Apollo Health City, Jubilee Hills, Hyderabad, Telangana 500033. India.