Journal of Research in Applied and Basic Medical Sciences

en The Histomorphometric Alterations in Male Sprague-Dawley Rats Following Exposure to Dextromethorphan and Antioxidants Administration Other Other پژوهشي orginal article Background  Morphometric evaluation of seminiferous tubules and epididymis is a common parameter used to assess male fertility. This study aimed to evaluate the morphological changes in male rats following exposure to dextromethorphan. Methods A total of 80 male rats (150 ± 30 g) were divided into four groups (N = 20; A-D) for the study. Group A received distilled water; group B received 20 mg/kg, group C received 40 mg/kg, and group D received 80 mg/kg of DM for a duration of four weeks. At the end of the treatment period, five rats were selected from each group and the following histomorphometric parameters were analyzed: diameter and height of the seminiferous tubule and epididymes, volume of testes, and the number of spermatogonia, spermatocytes, and spermatids within the seminiferous tubules. The remaining 15 rats were divided into three groups (N = 5; E-G). They received Rutin (25 mg/kg), Quercetin (50 mg/kg), and DW, respectively, for four weeks to ascertain the recovery rate. Results All histomorphometric parameters decreased compared to the control group; however, when comparing the recovery-alone group to the treatment groups, a slight increase was observed. The morphometric measurements showed an increase when Rutin and Quercetin were compared to both the treatment and recovery-alone groups. The comparison between Rutin and Quercetin showed no substantial difference in their effects. Conclusion Dextromethorphan has showed to have deleterious effect on the epididymal and testicular morphometry and this could culminate into infertility in males, however, these damages were significantly improved upon the administration of Rutin and Quercetin as antioxidants. Dextromethorphan, Histomorphometry, Rutin, Quercetin 271 279 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-503-1&slc_lang=en&sid=1 Oluwaseye Adesina Adebajo oluwaseye.adebajo@bowen.edu.ng 10031947532846005033 10031947532846005033 Yes Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Lagos, Nigeria Chinwe Stella Gbotolorun 10031947532846005034 10031947532846005034 No Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Lagos, Nigeria Ayodele Oremosu 10031947532846005035 10031947532846005035 No Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Lagos, Nigeria Perpetual Kuburat Adebajo 10031947532846005036 10031947532846005036 No Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Lagos, Nigeria Joshua Honor Ojo 10031947532846005037 10031947532846005037 No Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria Utom-Obong Udom Akpan 10031947532846005038 10031947532846005038 No Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria Zainab Adebusola Sogbesan 10031947532846005039 10031947532846005039 No Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Lagos, Nigeria Bassey Helen Akpan 10031947532846005040 10031947532846005040 No Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria Gloria Inioluwa Okegbemi 10031947532846005041 10031947532846005041 No Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria Jesulayomi Oluwayemisi Oladipo 10031947532846005042 10031947532846005042 No Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria Oyindamola Dorcas Akinwola 10031947532846005043 10031947532846005043 No Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria