Volume 8, Issue 4 (11-2022)
Journal of Research in Applied and Basic Medical Sciences 2022, 8(4): 211-220 |
Back to browse issues page
Assistant Professor, Department of Community Medicine, Diamond Harbour Medical College, West Bengal , subhro79@gmail.com
Abstract: (1005 Views)
Background & Aims: The immunohistological and ultrastructural features of the human testis with emphasis upon the process of spermatogenesis and the cytology of the Leydig cells were reviewed in this study. The present study also has its future implications in staging of cancer metastasis in the patients with Seminoma Testis and Leydig Cell Tumors and also in future xenografting of testicular tissue from an infant human donor.
Materials & Methods: The testis tissue samples from aborted human fetuses of various weeks of gestation were taken and then subjected to immunohistochemistry by Ki-67 antibodies and also to Scanning Electron Microscopy and Transmission Electron Microscopy.
Results: In the ultrastructural study, it is shown that the seminiferous epithelium is structurally partitioned by the Sertoli cells into basal and adluminal compartments via the specialized tight junctions between the Sertoli cells. The Leydig cell cytoplasm contains an abundant supply of smooth endoplasmic reticulum and mitochondria with tubular cristae, both features being characteristic of steroidogenic cells.
Conclusion: The detailed ultrastructural study can help the surgeons in the future xenografting processes of testicular tissue from an infant human donor to increase sperm maturity because of highly vascular testicular tissue.
Materials & Methods: The testis tissue samples from aborted human fetuses of various weeks of gestation were taken and then subjected to immunohistochemistry by Ki-67 antibodies and also to Scanning Electron Microscopy and Transmission Electron Microscopy.
Results: In the ultrastructural study, it is shown that the seminiferous epithelium is structurally partitioned by the Sertoli cells into basal and adluminal compartments via the specialized tight junctions between the Sertoli cells. The Leydig cell cytoplasm contains an abundant supply of smooth endoplasmic reticulum and mitochondria with tubular cristae, both features being characteristic of steroidogenic cells.
Conclusion: The detailed ultrastructural study can help the surgeons in the future xenografting processes of testicular tissue from an infant human donor to increase sperm maturity because of highly vascular testicular tissue.
Type of Study: orginal article |
Subject:
General
References
1. Riva A, Tandler B, Ushiki T, Usai P, Isola R, Conti G, Loy F, Hoppel CL. Mitochondria of human Leydig cells as seen by high resolution scanning electron microscopy. Arch Histol Cytol 2010;73(1):37-44. [DOI:10.1679/aohc.73.37] [PMID]
2. Miething A. Arrested germ cell divisions in the ageing human testis. Andrologia 2005;37(1):10-6 [DOI:10.1111/j.1439-0272.2004.00645.x] [PMID]
3. Miething A. Multinucleated spermatocytes in the aging human testis: formation, morphology, and degenerative fate. Andrologia 1993;25(6):317-23. [DOI:10.1111/j.1439-0272.1993.tb02733.x] [PMID]
4. Jiang H, Zhu WJ, Li J, Chen QJ, Liang WB, Gu YQ. Quantitative histological analysis and ultrastructure of the aging human testis. Int Urol Nephr 2014;46(5):879-85. [DOI:10.1007/s11255-013-0610-0] [PMID]
5. Johnson L, Petty CS, Neaves WB. Age-related variations in seminiferous tubules in men. A stereological evaluation. 1986 J Androl 7(5):316-22. [DOI:10.1002/j.1939-4640.1986.tb00939.x] [PMID]
6. Feinberg MJ, Lumia AR, McGinnis MY. The effect of anabolic-androgenic steroids on sexual behavior and reproductive tissues in male rats. Physiol Behav 1997;62(1):23-30. [DOI:10.1016/S0031-9384(97)00105-4] [PMID]
7. Blanco A, Flores-Acuna F, Roldan-Villalobos R, Monterde JG. Testicular damage from anabolic treatments with the β2-adrenergic agonist clenbuterol in pigs: a light and electron microscope study. Vet J 2002;163(3):292-8. [DOI:10.1053/tvjl.2001.0693] [PMID]
8. Gopalkrishnan K, Gill-Sharma MK, Balasinor N, Padwal V, D'Souza S, Parte P, Jayaraman S, Juneja HS. Tamoxifen-induced light and electron microscopic changes in the rat testicular morphology and serum hormonal profile of reproductive hormones. Contraception 1998;57(4):261-9. [DOI:10.1016/S0010-7824(98)00025-0] [PMID]
9. Johnson L. Spermatogenesis and aging in the human. J Androl 1986;7(6):331-54. [DOI:10.1002/j.1939-4640.1986.tb00943.x] [PMID]
10. Kerr JB. Functional cytology of the human testis. Baillieres Clin Endocrinol Metab 1992;6(2):235-50. [DOI:10.1016/S0950-351X(05)80149-1] [PMID]
11. Plas E, Berger P, Hermann M, Pfluger H. Effects of aging on male fertility? Exp Gerontol 2000;35(5):543-51. [DOI:10.1016/S0531-5565(00)00120-0] [PMID]
12. Richardson LL, Kleinman HK, Dym M. Altered basement membrane synthesis in the testis after tissue injury. J Androl 1998;19(2):145-55. [Google Scholar]
13. Santoro G, Romeo C, Impellizzeri P, Arco A, Rizzo G, Gentile C. A morphometric and ultrastructural study of the changes in the lamina propria in adolescents with varicocele. BJU Int 1999;83(7):828-3. [DOI:10.1046/j.1464-410x.1999.00023.x] [PMID]
14. Haider SG. Cell biology of Leydig cells in the testis. Int Rev Cytol 2004;233(4):181-241. [DOI:10.1016/S0074-7696(04)33005-6] [PMID]
15. Sato Y, Nozawa S, Yoshiike M, Arai M, Sasaki C, Iwamoto T. Xenografting of testicular tissue from an infant human donor results in accelerated testicular maturation. Hum Reprod 2010;25(5):1113-22. [DOI:10.1093/humrep/deq001] [PMID]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |