Journal of Research in Applied and Basic Medical Sciences
Journal of Research in Applied and Basic Medical Sciences
Medical Sciences
http://ijrabms.umsu.ac.ir
1
admin
2717-0098
8
10.61186/rabms
14
8888
13
en
jalali
1399
3
1
gregorian
2020
6
1
6
3
online
1
fulltext
en
The Effect of Human Amniotic Fluid in Decellularized Human Umbilical Vein Guide Channel on Sciatic Nerve Regeneration in Rats
The Effect of Human Amniotic Fluid in Decellularized Human Umbilical Vein Guide Channel on Sciatic Nerve Regeneration in Rats
تخصصي
Special
پژوهشي
orginal article
<strong><em>Background & Aims</em></strong>: The nerve autograft is the clinical gold standard in bridging nerve injury gaps, but it has severe disadvantages. The human umbilical vein (HUV) is suitable for multiple vascular reconstructive usages. The purpose of the present survey was to assess nerve regeneration by human amniotic fluid (AF) in the HUV channel.<br>
<strong><em>Materials&</em></strong> <strong><em>Methods:</em> </strong>In this study, 32 adult male rats (250-300g) were randomly divided into four groups: HUV+AF, HUV+normal saline (NS), Autograft, sham surgery. A centimeter gap in the sciatic nerve was grafted by autograft or HUV. Nerve regeneration was examined on days 28 and 90 after surgery by sciatic function index (SFI), electrophysiological assessments, histology, and immunohistology staining.<br>
<strong> <em>Results</em>:</strong> On days 60 and 90 after surgery, the SFI in the groups of autograft and HUV+AF was more than HUV+NS group (p <0.05). On the 90th day, the average nerve conduction velocity (NCV) and the number of myelinated axons in autograft and HUV+AF groups were significantly more than HUV+NS group (p <0.05).<br>
<strong><em>Conclusion</em></strong><strong>:</strong> The results of this study display that the HUV+AF may have beneficial effect for the treatment of peripheral nerve damages.
Nerve Regeneration, Human umbilical vein, Nerve guidance channel, Amniotic Fluid, Rat
144
152
http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-120-1&slc_lang=en&sid=1
Ghola Hossein
Farjah
hfarjah@hotmail.com
1003194753284600848
1003194753284600848
Yes
Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Parisa
Hadavi
hfarjah@hotmail.com
1003194753284600849
1003194753284600849
No
Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Masoumeh
Zirak Javanmard
hfarjah@hotmail.com
1003194753284600850
1003194753284600850
No
Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Bagher
Pourhydar
hfarjah@hotmail.com
1003194753284600851
1003194753284600851
No
Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Behnam
Heshmatiyan
hfarjah@hotmail.com
1003194753284600852
1003194753284600852
No
Department of physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Zahra
Nozhat
hfarjah@hotmail.com
1003194753284600853
1003194753284600853
No
Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran