en Detection of efflux pump gene cepA in Klebsiella pneumonia and its effect on resistance to biocides عمومى General پژوهشي orginal article <strong><em>Background & Aims</em></strong>: <em>Klebsiella pneumonia (K.pneumonia) </em>is one of the causative agents of lung infections, wound infections, urinary tract, and bloody diarrhea. One of the most common ways of transmission in neonatal and surgical wards is through hospital staff, nurses, and physicians. It could be transmitted to hospitalized patients and personnel through feces, respiratory secretions, contaminated equipment, and hands. To prevent the transmission of nosocomial infections, hand washing of employees with biocides can be effective.<span dir="RTL"></span><br> <strong><em>Materials & Methods:</em></strong> The minimum inhibitory concentration of 65 <em>K<span dir="RTL">.</span>pneumonia</em> isolates was determined according to CLSI guidelines compared to common biocides used in educational hospitals in Urmia, Iran, such as benzalkonium chloride and chlorhexidine. PCR was performed to evaluate the presence of <em>cep</em>A genes.<span dir="RTL"></span><br> <strong><em>Results:</em></strong> The results showed a significant relationship between the presence of <em>cep</em>A gene and high MIC compared to chlorhexidine bioside in <em>K<span dir="RTL">.</span> pneumoniae</em>. But there was no significant relationship between the presence of <em>cep</em>A gene and multidrug-resistant (MDR) isolates.<span dir="RTL"></span><br> <strong><em>Conclusion: </em></strong>It is concluded that, detection of <em>cep</em>A gene or other genes involving drug resistance should be extended by using another tests with more reliability and reproducibility like gene expressions and gene cloning methods. K.pneumonia, Chlorhexidine, MIC, cepA 135 138 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-58-2&slc_lang=en&sid=1 Shohre Afshar-Yavari shafsharyavari@yahoo.com 10031947532846001236 10031947532846001236 No Urmia University of Medical Sciences Kambiz Diba kambiz37diba@gmail.com 10031947532846001237 10031947532846001237 Yes Urmia University of Medical Sciences Sana Jabbari sanajabbari9@gmail.com 10031947532846001238 10031947532846001238 No Urmia University of Medical Sciences