Journal of Research in Applied and Basic Medical Sciences
Journal of Research in Applied and Basic Medical Sciences
Medical Sciences
http://ijrabms.umsu.ac.ir
1
admin
2717-0098
8
10.61186/rabms
14
8888
13
en
jalali
1399
12
1
gregorian
2021
3
1
7
1
online
1
fulltext
en
Topical application of Dianthus essential oil improved the infected healing process of wounds infected with Staphylococcus aureus in an experimental model
Topical application of Dianthus essential oil improved the infected healing process of wounds infected with Staphylococcus aureus in an experimental model
تخصصي
Special
پژوهشي
orginal article
<strong><em>Background & Aims</em></strong>: In recent years, wound infections caused by <em>Staphylococcus aureus</em> have substantially grown. Lack of easy access, medications expensive, side effects, and in particular the development of drug resistance, the use of biological materials are proposed as an alternative solution. The recent study was aimed to evaluate the topical effect of <em>Dianthus </em>essential oil on cutaneous excisional wound healing in mice infected with <em>S. aureus</em>.<br>
<strong><em> Materials & Maethods:</em></strong> This study was performed on 36 mice (weight 25±3 g). After general anesthesia, 0.5 mm circle wound was created with biopsy punch between the shoulder, and immediately 50 γ of the suspension containing 10<sup>7</sup> CFU/ml <em>S. Aureus</em> was applied to the wound. Then tested animals, grouping in three groups of 12 mice each (control, treated with ointment 2% and 4%). During the project, image was obtained on days 3, 6, 9 and 12 images for assessment of wound area, and in 3, 7 and 14 from wounds in order to histopathology assessment.<br>
<strong><em>Results</em></strong>: The results of the wound size showed that the wound area decreased significantly in the treated groups (p< 0.05) compared with the control group. On histological examination, a significant (p< 0.05) reduction was observed in the migration of immune cells, the migration of fibroblasts and fibrocytes into the wound, the collagen synthesis and secretion. In addition, the thickness of the epithelium thickest increased in the treatment groups compared with the control group (p< 0.05).<br>
<strong><em>Conclusion</em></strong>: Based on the results of this study, topical application of <em>Dianthus</em> essential oil, especially at higher therapeutic doses, can be considered a viable option for treatment of infected wounds by the bacteria <em>S. aureus</em>.<span dir="RTL"></span>
Dianthus essential oil, infected wound healing, Staphylococcus Aureus, ointment, mice
18
25
http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-1-7&slc_lang=en&sid=1
Shiva
Refahi Rezaie
shrumsu.ac.ir
10031947532846001076
10031947532846001076
Yes
Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran
Mohammad Hossein
Sadeghi Zali
shrumsu.ac.ir
10031947532846001077
10031947532846001077
No
Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran
Mohammadreza
Farahpour
shrumsu.ac.ir
10031947532846001078
10031947532846001078
No
Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran
Aynaz
Mihanfar
shrumsu.ac.ir
10031947532846001079
10031947532846001079
No
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran