en Topical application of Dianthus essential oil improved the infected healing process of wounds infected with Staphylococcus aureus in an experimental model تخصصي Special پژوهشي orginal article <strong><em>Background & Aims</em></strong>: &nbsp;In recent years, wound infections caused by <em>Staphylococcus aureus</em> have substantially grown. Lack of easy access, medications expensive, side effects, and in particular the development of drug resistance, the use of biological materials are proposed as an alternative solution. The recent study was aimed to evaluate the topical effect of <em>Dianthus </em>essential oil on cutaneous excisional wound healing in mice infected with <em>S. aureus</em>.<br> <strong><em>&nbsp;Materials & Maethods:</em></strong> This study was performed on 36 mice (weight 25&plusmn;3 g). After general anesthesia, 0.5 mm circle wound was created with biopsy punch between the shoulder, and immediately 50 &gamma; of the suspension containing 10⁷ CFU/ml <em>S. Aureus</em> was applied to the wound. Then tested animals, grouping in three groups of 12 mice each (control, treated with ointment 2% and 4%). During the project, image was obtained on days 3, 6, 9 and 12 images for assessment of wound area, and in 3, 7 and 14 from wounds in order to histopathology assessment.<br> <strong><em>Results</em></strong>: The results of the wound size showed that the wound area decreased significantly in the treated groups (p< 0.05) compared with the control group. On histological examination, a significant (p< 0.05) reduction was observed in the migration of immune cells, the migration of fibroblasts and fibrocytes into the wound, the collagen synthesis and secretion. In addition, the thickness of the epithelium thickest increased in the treatment groups compared with the control group (p< 0.05).<br> <strong><em>Conclusion</em></strong>: Based on the results of this study, topical application of <em>Dianthus</em> essential oil, especially at higher therapeutic doses, can be considered a viable option for treatment of infected wounds by the bacteria <em>S. aureus</em>.<span dir="RTL"></span> Dianthus essential oil, infected wound healing, Staphylococcus Aureus, ointment, mice 18 25 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-1-7&slc_lang=en&sid=1 Shiva Refahi Rezaie shrumsu.ac.ir 10031947532846001076 10031947532846001076 Yes Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran Mohammad Hossein Sadeghi Zali shrumsu.ac.ir 10031947532846001077 10031947532846001077 No Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran Mohammadreza Farahpour shrumsu.ac.ir 10031947532846001078 10031947532846001078 No Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran Aynaz Mihanfar shrumsu.ac.ir 10031947532846001079 10031947532846001079 No Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran