en Evaluation the Anti-proliferative Effect of NVP-AUY922 in Combination with Thymoquinone in Colorectal Cancer Cell Lines عمومى General پژوهشي orginal article <strong><em>Background & Aims</em></strong>: &nbsp;Thymoquinone (TQ) as a natural component and the active herbal complex originate in <em>Nigella sativa</em> seed shows the anti-cancer effects acknowledged in the previous studies. The effects of TQ, its mechanism on colorectal cancer, and its combination with other newly chemotherapeutic agents are unclear. Heat shock protein 90 (HSP90) has been upregulated in a number of malignancies. In this survey, we have investigated the impacts of Thymoquinone and NVP-AUY922 (a newly defined resorcinylic isoxazole&ndash;based HSP90 inhibitor) on HT-29 colorectal cancer cell lines.<br> <strong><em>Materials & Methods: </em></strong>HT-29 cells were seeded and exposed to Thymoquinone and NVP-AUY922 for 24 hours in various concentrations. Cell viability (water-soluble tetrazolium-1) assay was performed. Moreover, in combination cases, various concentrations of both agents examined using cellular viability analysis.<br> <strong><em>Results:</em></strong> The TQ significantly inhibited cancer cell growth in colorectal cancer cell lines in various combination doses of NVP-AUY922. Treatment with TQ could augment the cytotoxicity of NVP-AUY922 against the HT-29 as compared with that of NVP-AUY922 alone.<br> <strong><em>Conclusion</em></strong>: Our findings suggested the anti-proliferative effects of TQ and NVP-AUY922 through cytotoxic pathway to induce cell death. NVP-AUY922, Thymoquinone, Colorectal Cancer Cell Lines 122 127 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-158-1&slc_lang=en&sid=1 Attabak Toofani milani toofani2020@yahoo.com 10031947532846001228 10031947532846001228 Yes Department of Biochemistry, Medicine Faculty, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran. Mahshid Mohammadian Biochem426@gmail.com 10031947532846001229 10031947532846001229 No Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Urmia University of Medical Sciences, Urmia, Iran. Roghayeh Paribananaem r.paribanaem69@gmail.com 10031947532846001230 10031947532846001230 No Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran