en تخصصي Special پژوهشي orginal article <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Background & Aims</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span style="font-size:9.0pt">Chronic human myeloid leukemia (CML) is caused by mutations and changes in stem cells. This study aimed to investigate the toxicity, apoptosis, and cell cycle of thiosemicarbazone complex with copper on the human chronic myelogenous K562 leukemia cell line.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Materials & Methods</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> &nbsp;After culturing the human K562 cell line, it was exposed to the combination of methyl thiosemicarbazone complex with copper in different concentrations and durations. Trypan blue dye exclusion test and MTT were used to determine cell viability and cell growth inhibition. The occurrence of apoptosis was examined by dual acridine orange/ethidium bromide (AO/EB) fluorescent staining and fluorescence microscopy, cell cycle analysis, and dual PI/AnnexinV staining using flow cytometry.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Results</span></i></b><b><span style="font-size:9.0pt">:</span></b><span style="font-size:9.0pt"> &nbsp;The data obtained from the present study showed morphological changes resulting from apoptosis and cell cycle arrest in Sub G1 in the presence of phosphatidylserine in the outer leaflet of the cell membrane due to treatment with thiosemicarbazone compound. It also decreased the biological growth of the K562 cell line in a concentration-/ and time-dependent manner.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:16.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><i><span style="font-size:9.0pt">Conclusion</span></i></b><b><span style="font-size:9.0pt">: </span></b>&nbsp;<span style="font-size:9.0pt">effective at low concentrations and short duration of action, this compound can be a suitable candidate for future pharmacological studies on treating CML.</span></span></span></span> Chronic human myeloid leukemia, Cytotoxic Effects, K562, Pharmacological Studies, Thiosemicarbazone 228 236 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-215-1&slc_lang=en&sid=1 Neda Kazemi Motlagh nedakazemi72@gmail.com 10031947532846001712 10031947532846001712 No Department of Microbial Biotechnology, Tehran Center Branch, Islamic Azad University, Tehran, Iran Saeed Hesami Tackallou tackallou@gmail.com 10031947532846001713 10031947532846001713 No Department of Microbial Biotechnology, Tehran Center Branch, Islamic Azad University, Tehran, Iran Majid Mahdavi maj.mahdavi@gmail.com 10031947532846001714 10031947532846001714 No Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Mahdi Hosseinzadeh Kevin.Hosseinzadeh@gmail.com 10031947532846001715 10031947532846001715 Yes Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran