en تخصصي Special پژوهشي orginal article <p dir="ltr"><strong>Received 7 May 2015, accepted for publication 29 July 2015</strong></p> <p dir="ltr"><strong>Background & Aims: </strong>Anticancer drugs are known to have great impact on spermatogenesis process and germinal epithelium. The present study is to investigate the preventive effect of Cetrorelix (GnRH antagonist) on Cyclophosphamide- induced spermatogenic defect.</p> <p dir="ltr"><strong>Methods and Materials: </strong>Fifty adult female mice aging 6-8 weeks (30&plusmn;4 gr) were divided into 3 groups as: Control, Experimental 1 and Experimental 2. The animals in Experimental 1 group received Cyclophosphamide (2.5 mg/kg, ip) for 5 days and in Experimental 2 group received Cetrorelix (0.25 mg/kg, ip) one week before Cyclophosphamide treatment and continued for 3 weeks. The mice in all groups were sacrificed 35 days after the last injection. Ovarian follicles were isolated mechanically and the viability was studied by trypan blue staining.</p> <p dir="ltr"><strong>Results: </strong>Ovarian follicles of animals in Experimental 2group, retained higher percentage of normal morphology (P<0.001) than which in Experimental 1 group and their condition were similar to control group.</p> <p dir="ltr"><strong>Conclusion: </strong>These results indicate that the Cetrorelix administration before cancer treatment may protect ovarian tissue against side effects of cisplatin.</p> <p dir="ltr"><strong>Keywords: </strong>Ovarian follicles, Viability, GnRH antagonist, Cetrorelix</p> Ovarian follicles, Viability, GnRH antagonist, Cetrorelix 56 60 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-55-2&slc_lang=en&sid=1 Daryosh Mohammadnejad abedelahia@yahoo.com 1003194753284600255 1003194753284600255 No Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Hamid Tayefi-nasrabadi abedelahia@yahoo.com 1003194753284600256 1003194753284600256 No Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran Mehran Naghibi abedelahia@yahoo.coma 1003194753284600257 1003194753284600257 No Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran Ali Abedelahi abedelahia@yahoo.com 1003194753284600258 1003194753284600258 Yes Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran