en Investigating the effect of Sclareol on IRE-1 and PERK genes the pathway of reticulandaplasmic system stress in gastric cancer cells MKN-45 تخصصي Special پژوهشي orginal article <strong><em>Background & Aims</em></strong>: &nbsp;Despite the decline in the prevalence of gastric cancer in recent years, it remains the fourth leading cause of death from cancer in the world. Common cancer treatments may reduce the size of the tumor, but it is transient and does not have a positive effect on the patient&#39;s survival and there is a possibility of recurrence of the disease. Strong induction of reticulom endoplasmic has been shown to increase the susceptibility to anti-cancer therapy. Regarding the importance of medicinal herbs in recent years and its low side effects after administration, compared with synthetic drugs, this study investigated the effects of salvia Sclareol purification from sage on the induction of reticulum endoplasmic system stress.<br> <strong><em>Materials and Methods</em></strong>: The MKN-45 cell line from the Pasteur Institute of Iran was purchased and cultured in a complete culture medium of RPMI-1640 with cetacean embryos. Cells cultured with 0, 20, 40, 60, 80 and 100 &mu;m concentrations of Sclareol treatment for 5 hours. The rate of expression of IRE-1 and PERK genes by quantitative real time -PCR and the level of proteins IRE-1 and PERK by western blotting method was investigated.<br> <strong><em>Results</em></strong>: The rate of expression of IRE-1 in doses of 20, 40 and 60 &mu;M Sclareol was significantly increased while decreasing in doses of 80 and 100 &mu;M (p <0.0001). Also, the expression of PERK gene expression at doses of 20, 40 and 60 &mu;M Sclareol was significantly increased, but no increase was observed in doses of 80 and 100 &mu;M (p <0.0001). Also, the levels of IRE-1 and PERK proteins in doses of 20, 40 and 60 micromoles of Sclareol showed a high increase in doses of 80 and 100 &mu;M.<br> <strong><em>Conclusion</em></strong>: From the results of this study, it seems that doses between 20 and 60 &mu;mol of can be Sclareol helpful in increasing the amount of reticuloendoplasmic stress, but doses higher than 60 milimoles do not have like this effect.<br> &nbsp; gastric Cancer, Sclareol, reticuloendoplasmic system stress 44 32 http://ijrabms.umsu.ac.ir/browse.php?a_code=A-10-101-10&slc_lang=en&sid=1 Elmira Aboutalebi monavvar03@gmail.com 1003194753284600723 1003194753284600723 No MSc Student in Genetics, Dept. of Biology, Allameh Amini Faculty of Basic Sciences, Azad Islamic University, Tabriz Branch, Tabriz, East Azarbaijan, Iran Ebrahim Sakhinia monavvar03@gmail.com 1003194753284600724 1003194753284600724 No MSc Student in Genetics, Dept. of Biology, Allameh Amini Faculty of Basic Sciences, Azad Islamic University, Tabriz Branch, Tabriz, East Azarbaijan, Iran Vand Beilanokhi 1003194753284600725 1003194753284600725 No Associate Professor in Human Genetics, Dept. of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, East Azarbaijan, Iran Homayoun Dolatkhah dolatkhahh@gmail.com 1003194753284600726 1003194753284600726 Yes PhD in Clinical Biochemistry, Dept. of Clinical Biochemistry and Laboratories Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, East, Azarbaijan, Iran