Biochemical assessments of effect of nefidipne on ischemia-reperfusion injury in rat ovaries via systemic administration

Velayati, Mohammad; Rahimzadeh, Hakim; Malekolkalami, Mozhgani; Shamsi, Hamid; Khanbaba, Gholamreza

Volume 6, Issue 2 (April 2020) Journal of Research in Applied and Basic Medical Sciences 2020, 6(2): 79-89 | Back to browse issues page

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Velayati M, Rahimzadeh H, Malekolkalami M, Shamsi H, Khanbaba G. Biochemical assessments of effect of nefidipne on ischemia-reperfusion injury in rat ovaries via systemic administration. Journal of Research in Applied and Basic Medical Sciences 2020; 6 (2) :79-89
URL: http://ijrabms.umsu.ac.ir/article-1-104-en.html

Biochemical assessments of effect of nefidipne on ischemia-reperfusion injury in rat ovaries via systemic administration

Mohammad Velayati

, Hakim Rahimzadeh

, Mozhgani Malekolkalami

, Hamid Shamsi

, Gholamreza Khanbaba ^*

Department of Clinical Sciences. Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran , gholamreza.khanbab@gmail.com

Abstract: (2656 Views)

Background & Aims: Different conditions like long mesovarium and adnexal venous congestion could result in torsion of ovary and subsequently obstruction of the ovarian vessels. The aim of the present study was to investigate effects of systemic administration of nefidipine on ischemia-reperfusion injury in ovaries.
Materials and Methods: Twenty-five healthy female Wistar rats about ~250g were randomized into five experimental groups (n = 5): Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. Group I/N: A 3-hour ischemia only and 100 mg/kg intraperitoneal administration (IP) of nefidipine 2.5 hours after induction of ischemia. Group I/R/N: A 3-hour ischemia, a 3-hour reperfusion and 100 mg/kg IP of nefidipine 2.5 hours after induction of ischemia.
Results: Animals treated with nefidipine showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (P<0.05). The significant higher values of SOD, tGSH, GPO, GSHRd and GST were observed in I/R/NC animals compared to those of other groups (P<0.05). Damage indicators (NOS, MDA, MPO and DNA damage level) were significantly lower in I/R/NC animal compared to those of other groups (P<0.05).
Discussion: Intraperitoneal administration of nefidipine could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.

Keywords: Ischemia-reperfusion, nefidipine, interaperitoneal, ovary

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Type of Study: orginal article | Subject: General

References

1. Oelsner G, Shashar D. Adnexal torsion. Clin Obstet Gynecol 2006;49(3):459-63. [DOI:10.1097/00003081-200609000-00006] [PMID]

2. Geimanaite L, Trainavicius K. Ovarian torsion in children: management and outcomes. J Pediatr Surg 2013;48(9):1946-53. [DOI:10.1016/j.jpedsurg.2013.04.026] [PMID]

3. Celik A, Ergün O, Aldemir H, Ozcan C, Ozok G, Erdener A, et al. Long-term results of conservative management of adnexal torsion in children. J Pediatr Surg 2005;40(4):704-8. [DOI:10.1016/j.jpedsurg.2005.01.008] [PMID]

4. Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol 2000;190(3):255-66. https://doi.org/10.1002/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6 [DOI:10.1002/(SICI)1096-9896(200002)190:33.0.CO;2-6]

5. Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am 1992;72(1):65-83. [DOI:10.1016/S0039-6109(16)45628-8]

6. Nakagiri A, Sunamoto M, Takeuchi K. Evidence for the involvement of NADPH oxidase in ischemia/reperfusion-induced gastric damage via angiotensin II. J Physiol Pharmacol 2010;61(2):171-9. [Google Scholar]

7. Javanmardi S, Khordadmehr M. Benidipine reduces ischemia/reperfusion injury following testicular torsion/detorsion in rats. Iranian J Vet Surg 2017;12(2):21-7. [Google Scholar]

8. Ingec M, Isaoglu U, Yilmaz M, Calik M, Polat B, Alp HH, et al. Prevention of ischemia-reperfusion injury in rat ovarian. J Physiol Pharmacol 2011;62(5):575-82. [PubMed]

9. Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia- reperfusion injury. Mol Aspects Med 2004;25(1-2):199-210. [DOI:10.1016/j.mam.2004.02.020] [PMID]

10. Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. J Lipid Res 1998;39(8):1529-42. [DOI:10.1016/S0022-2275(20)32182-9]

11. Huang HY, Helzlsouer KJ, Appel LJ. The effects of vitamin C and vitamin E on oxidative DNA damage: results from a randomized controlled trial. Cancer Epidemiol Biomarkers Prev 2000;9(7):647-52. [PubMed]

12. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993;90(17):7915-22. [DOI:10.1073/pnas.90.17.7915] [PMID] [PMCID]

13. Oral A, Odabasoglu F, Halici Z, Keles ON, Unal B, Coskun AK, et al. Protective effects of montelukast on ischemia-reperfusion injury in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Fertil Steril 2011;95(4):1360-6. [DOI:10.1016/j.fertnstert.2010.08.017] [PMID]

14. Halici Z, Karaca M, Keles ON, Borekci B, Odabasoglu F. Protective effects of amlodipine on ischemiareperfusion injury of rat ovary: biochemical and histopathologic evaluation. Fertil Steril 2008;90:2408-15. [DOI:10.1016/j.fertnstert.2007.10.007] [PMID]

15. Lindsay RW, Heaton JT, Edwards C, Smitson C, Hadlock TA. Nimodipine and Acceleration of Functional Recovery of the Facial Nerve After Crush Injury. Arch Facial Plast Surg 2010;12:49-52. https://doi.org/10.1001/archfaci.2009.95 [DOI:10.1001/archfacial.2009.95] [PMID] [PMCID]

16. Kulah B, Besler HT, Akdag M, Oruc T, Altinok G, et al. The effects of verapamil vs. allopurinol on intestinal ischemia/reperfusion injury in rats. An experimental study. Hepatogastroenterology 2004;51:401-7. [PubMed]

17. Bramley PM, Elmadfa I, Kafatos A, Kelly FJ, Manios Y, Roxborough HE, et al. Vitamin E. J Sci Food Agric 2000;80(7):913-38. https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<913::AID-JSFA600>3.0.CO;2-3 [DOI:10.1002/(SICI)1097-0010(20000515)80:73.0.CO;2-3]

18. Farias MC, Moura ML, Andrade L, Leão MH. Encapsulation of the alpha-tocopherol in a glassy food model matrix. Mate Res 2007;10(1):57-62. [DOI:10.1590/S1516-14392007000100013]

19. Hategekimana J, Masamba KG, Ma J, Zhong F. Encapsulation of vitamin E: effect of physicochemical properties of wall material on retention and stability. Carbohydr Polym 2015;124(1):172-9. [DOI:10.1016/j.carbpol.2015.01.060] [PMID]

20. Trombino S, Cassano R, Muzzalupo R, Pingitore A, Cione E, Picci N. Stearyl ferulate-based solid lipid nanoparticles for the encapsulation and stabilization of β-carotene and nefidipine. Colloids Surf B Biointerfaces 2009;72(2): 181-7. [DOI:10.1016/j.colsurfb.2009.03.032] [PMID]

21. Duclairoir C, Orecchioni A, Depraetere P, Nakache E. nefidipine encapsulation and in vitro release from wheat gliadin nanoparticles. J Microencapsul 2002;19(1):53-60. [DOI:10.1080/02652040110055207] [PMID]

22. Quiñones JP, Gothelf KV, Kjems J, Yang C, Caballero AMH, Schmidt C, et al. Self-assembled nanoparticles of modified-chitosan conjugates for the sustained release of dl-nefidipine. Carbohydr Polym 2013;92(1):856-64. [DOI:10.1016/j.carbpol.2012.10.005] [PMID]

23. Luo Y, Zhang B, Whent M, Yu L, Wang Q. Preparation and characterization of zein/chitosan complex for encapsulation of nefidipine, and its in vitro controlled release study. Colloids Surf B Biointerfaces 2011;85(2):145-52. [DOI:10.1016/j.colsurfb.2011.02.020] [PMID]

24. Cortés-Sanabria L, Paredes-Ceseña CA, Herrera-Llamas RM, Cruz-Bueno Y, Soto-Molina H, Pazarín L, et al. Comparison of cost-utility between automated peritoneal dialysis and continuous ambulatory peritoneal dialysis. Arch Med Res 2013;44(8):655-61. [DOI:10.1016/j.arcmed.2013.10.017] [PMID]

25. Chaudhary K, Haddadin S, Nistala R, Papageorgio C. Intraperitoneal Drug Therapy: An Advantage. Curr Clin Pharmacol 2010;5(2):82-8. [DOI:10.2174/157488410791110779] [PMID]

26. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983;16(2):109-10. [DOI:10.1016/0304-3959(83)90201-4]

27. Sun Y, Larry WO, Ying L. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988;34(3):497-500. [DOI:10.1093/clinchem/34.3.497] [PMID]

28. Feelisch M, Noack EA. Correlation between nitric oxide formation during degradation of organic nitrates and activation of guanylate cyclase. Eur J Pharmacol 1987;139(1):19-30. [DOI:10.1016/0014-2999(87)90493-6]

29. Knowles RG, Merrett M, Salter M, Moncada S. Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. Biochem J 1990;270(3):833-6. [DOI:10.1042/bj2700833] [PMID] [PMCID]

30. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8. [DOI:10.1016/0003-2697(79)90738-3]

31. Wei H, Frenkel K. In vivo formation of oxidized DNA bases in tumor promoter-treated mouse skin. Cancer Res 1991;51(16):4443-9. [PubMed]

32. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968;25(1):192-205. [DOI:10.1016/0003-2697(68)90092-4]

33. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. 1976. Biochem Biophys Res Commun 2012;425(3):503-9. [DOI:10.1016/j.bbrc.2012.08.016] [PMID]

34. Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985;113:484-90. [DOI:10.1016/S0076-6879(85)13062-4]

35. Habig WH, Jakoby WB. Assays for differentiation of glutathione S-transferases. Methods Enzymol. 1981;77:398-405. [DOI:10.1016/S0076-6879(81)77053-8]

36. Celik O, Turkoz Y, Hascalik S, Hascalik M, Cigremis Y, Mizrak B, et al. The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 2004;117(2):183-8. [DOI:10.1016/j.ejogrb.2004.05.007] [PMID]

37. Arosio B, Gagliano N, Fusaro LM, Parmeggiani L, Tagliabue J, Galetti P, et al. Aloe-Emodin quinone pretreatment reduces acute liver injury induced by carbon tetrachloride. Pharmacol Toxicol 2000;87(5):229-33. https://doi.org/10.1034/j.1600-0773.2000.d01-79.x [DOI:10.1034/j.1600-0773.2000.pto870507.x] [PMID]

38. Chatterjee PK, Patel NS, Kvale EO, Cuzzocrea S, Brown PA, Stewart KN, et al. Inhibition of inducible nitric oxide synthase reduces renal ischemia/reperfusion injury. Kidney Int 2002;61(3):862-71. [DOI:10.1046/j.1523-1755.2002.00234.x] [PMID]

39. Jeddi S, Zaman J, Zadeh-Vakili A, Zarkesh M, Ghasemi A. Involvement of inducible nitric oxide synthase in the loss of cardioprotection by ischemic postconditioning in hypothyroid rats. Gene 2016;580(2):169-76. [DOI:10.1016/j.gene.2016.01.014] [PMID]

40. Ferdinandy P, Schulz R. Nitric oxide, superoxide, and peroxynitrite in myocardial ischaemia-reperfusion injury and preconditioning. Br J Pharmacol 2003;138(4):532-43. [DOI:10.1038/sj.bjp.0705080] [PMID] [PMCID]

41. Valko M, Morris H, Cronin MT. Metals, toxicity and oxidative stress. Curr Med Chem 2005;12(10):1161-208. [DOI:10.2174/0929867053764635] [PMID]

42. Niki E, Yoshida Y, Saito Y, Noguchi N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun 2005;338(1):668-76. [DOI:10.1016/j.bbrc.2005.08.072] [PMID]

43. Ximenes VF, Paino IM, Faria-Oliveira OM, Fonseca LM, Brunetti IL. Indole ring oxidation by activated leukocytes prevents the production of hypochlorous acid. Braz J Med Biol Res 2005;38(11):1575-83. [DOI:10.1590/S0100-879X2005001100003] [PMID]

44. Van Antwerpen P, Boudjeltia KZ, Babar S, Legssyer I, Moreau P, Moguilevsky N, et al. Thiol-containing molecules interact with the myeloperoxidase/H2O2/chloride system to inhibit LDL oxidation. Biochem Biophys Res Commun 2005;337(1):82 -8. [DOI:10.1016/j.bbrc.2005.09.013] [PMID]

45. Isaoglu U, Yılmaz M, Sener E, Cetin N, Altuner D,Bilen H, et al. The impaired balances of oxidant/antioxidant and COX-1/COX-2 in ovarian ischemia-reperfusion injury and prevention by nimesulide. Lat Am J Pharm 2012;31(10):1481-8. [Google Scholar]

46. Meister A. Glutathione deficiency produced by inhibition of its synthesis, and its reversal; applications in research and therapy. Pharmacol Ther 1991;51(2):155-94. [DOI:10.1016/0163-7258(91)90076-X]

47. Celebi F, Akbas A, Saglam MB. Effect of sertraline in indomethacin-induced gastric mucosal damage. Asian J Chem 2012;24(5):1966-70. [Google Scholar]

48. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160(1):1-40. [DOI:10.1016/j.cbi.2005.12.009] [PMID]

49. Sharma H, Zhang X, Dwivedi C.The effect of ghee (clarified butter) on serum lipid levels and microsomal lipid peroxidation. Ayu 2010;31(2):134-40. [DOI:10.4103/0974-8520.72361] [PMID] [PMCID]

50. Polat B, Suleyman H, Alp HH. Adaptation of rat gastric tissue against indomethacin toxicity. Chem Biol Interact 2010;186(1):82-9. [DOI:10.1016/j.cbi.2010.03.041] [PMID]

51. Shi HY, Li ZH, Zhang YX, Chen L, Xiang DY, Zhang YF. Two pear glutathione S-transferases genes are regulated during fruit development and involved in response to salicylic acid, auxin, and glucose signaling. PLoS One 2014;9(2):e89926. [DOI:10.1371/journal.pone.0089926] [PMID] [PMCID]

52. Mansoorali KP, Prakash T, Kotresha D, Prabhu K, Rama Rao N. Cerebroprotective effect of Eclipta alba against global model of cerebral ischemia induced oxidative stress in rats. Phytomedicine 2012;19(12):1108-16. [DOI:10.1016/j.phymed.2012.07.004] [PMID]

53. Milligan JR, Aguilera JA, Nguyen TT, Ward JF, Kow YW, He B, et al. Yield of DNA strand breaks after base oxidation of plasmid DNA. Radiat Res 1999;151(3):334-42. [DOI:10.2307/3579946] [PMID]

54. Grollman AP, Moriya M. Mutagenesis by 8-oxoguanine: an enemy within. Trends Genet 1993;9(7):246-9. [DOI:10.1016/0168-9525(93)90089-Z]

55. Aksoy AN. Ovarian Ischemia-Reperfusion Injury: A Brief Review. SM J Gynecol Obstet 2015;1:1008-111.

56. Srivastava G, Mehta JL. Currying the heart: curcumin and cardioprotection. J Cardiovasc Pharmacol Ther 2009; 14(1):22e7. [DOI:10.1177/1074248408329608] [PMID]

57. Uguralp S, Bay Karabulut A, Mizrak B. Effects of pentoxifylline and vitamin E on the bilateral ovary after experimental ovarian ischemia. Eur J Pediatr Surg 2005;15(2):107-13. [DOI:10.1055/s-2004-821256] [PMID]

58. Nugent D, Newton H, Gallivan L, Gosden RG. Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. J Reprod Fertil 1998;114:341-6. [DOI:10.1530/jrf.0.1140341] [PMID]

59. Turner PV, Brabb T, Pekow C, Vasbinder MA. Administration of Substances to Laboratory Animals: Routes of Administration and Factors to Consider. J Am Assoc Lab Anim Sci 2011;50(5): 600-13 [PubMed]