XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hossein Somi M, Dolatkhah H, Movahedian A, Aghazade A M, Esfahani A, Dolatkhah N et al . The effect of omega fatty acids oral administration on apoptotic regulatory proteins in patients with gastric adenocarcinoma under chemotherapy-A double blind clinical trial study. Journal of Research in Applied and Basic Medical Sciences 2020; 6 (3) :123-133
URL: http://ijrabms.umsu.ac.ir/article-1-112-en.html
Professor in Liver and Gastrointestinal Disease, Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz,Iran , mhosseinsina@yahoo.com
Abstract:   (2342 Views)
Background & Aims:  The use of some active factors in diet is regarded as an attractive approach to prevent and to treat certain types of cancers. Accordingly, the main objective of this study was to evaluate the effect of PUFAs oral administration along with chemotherapeutic agent on the level of cellular apoptotic regulatory proteins in cancer cells of individuals with gastric cancer in order to identify the apoptotic changes.
Materials & Methods: This study was a Clinical Trial in which the target group consisted of the patients with gastric cancer who were recognized for the first time and cured under chemotherapy. Thirty-four patients were chosen and categorized randomly into two groups. Case group includes the patients taking PUFAs along with the chemotherapeutic agents. In control group, individuals were under the same chemotherapy protocol without taking PUFAs. Biopsy samples of tumor were taken from the patients before and after chemotherapy. The Bcl-2, Bcl-XL Bid, and Bad gene expression were determined by Real-Time PCR. Also, those proteins upon biopsy samples were surveyed by Frozen Section method.
Results: In case group, Bcl-2 and BclXL gene expression and protein levels decreased significantly in comparison with those of the control group. While Bid and Bad gene expression and protein levels increased significantly in comparison with those of the control group.
Conclusion: The results of this study showed that use of PUFAs as supplement with Cis-platinum may be useful to stimulate more pro-apoptotic proteins in gastric cancer cells. Consequently, this offers an effective treatment to patients with gastric cancer to respond to chemotherapy.
Full-Text [PDF 560 kb]   (1223 Downloads)    
Type of Study: orginal article | Subject: Special

1. Movahedian A, Rahbani-Nobar M, Dolatkhah N, Mortazavi M, Dolatkhah H. Effect of omega 3 fatty acids on serum lipid profile, malondialdehyde and paraoxonase activity in patients with end stage renal disease under regular hemodialysis. Res Pharmaceutical Sci 2012;7(5):914. [google scholar]
2. Dolatkhah H, Movahedian A, Somi MH, Aghaei M, Samadi N, Mirza-Aghazade A, et al. Effect of PUFAs Oral Administration on the Amount of Apoptotic Caspases Enzymes in Gastric Cancer Patients Undergoing Chemotherapy. Anti-cancer Agents Med Chem 2017;17(1):93-101. [DOI:10.2174/1871520616666160520113503]
3. Fenton JI, Hord NG, Ghosh S, Gurzell EA. Immunomodulation by dietary long chain omega-3 fatty acids and the potential for adverse health outcomes. Prostaglandins Leukot Essent Fatty Acids 2013;89(6):379-90. [DOI:10.1016/j.plefa.2013.09.011]
4. Ferro A, Peleteiro B, Malvezzi M, Bosetti C, Bertuccio P, Levi F, et al. Worldwide trends in gastric cancer mortality (1980-2011), with predictions to 2015, and incidence by subtype. Eur J Cancer 2014;50(7):1330-44. [DOI:10.1016/j.ejca.2014.01.029]
5. Dicken BJ, Bigam DL, Cass C, Mackey JR, Joy AA, Hamilton SM. Gastric adenocarcinoma: review and considerations for future directions. Ann Surg 2005;241(1):27-39. [DOI:10.1097/01.sla.0000149300.28588.23]
6. Yang D, Hendifar A, Lenz C, Togawa K, Lenz F, Lurje G, et al. Survival of metastatic gastric cancer: Significance of age, sex and race/ethnicity. J Gastrointest Oncol 2011;2(2):77-84. [PubMed]
7. Hamzeloo-Moghadam M, Aghaei M, Fallahian F, Jafari SM, Dolati M, Abdolmohammadi MH, et al. Britannin, a sesquiterpene lactone, inhibits proliferation and induces apoptosis through the mitochondrial signaling pathway in human breast cancer cells. Tumour Biol 2015;36(2):1191-8. [DOI:10.1007/s13277-014-2744-9]
8. Huang H, Han Y, Zhang C, Wu J, Feng J, Qu L, et al. HNRNPC as a candidate biomarker for chemoresistance in gastric cancer. Tumour Biol 2016;37(3):3527-34. [DOI:10.1007/s13277-015-4144-1]
9. Yu LL, Wu JG, Dai N, Yu HG, Si JM. Curcumin reverses chemoresistance of human gastric cancer cells by downregulating the NF-kappaB transcription factor. Oncol Rep 2011;26(5):1197-203. [DOI:10.3892/or.2011.1410]
10. Zhang D, Fan D. New insights into the mechanisms of gastric cancer multidrug resistance and future perspectives. Future Oncol 2010;6(4):527-37. [DOI:10.2217/fon.10.21]
11. Li K, Sun Z, Zheng J, Lu Y, Bian Y, Ye M, et al. In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer. J Proteomics 2013;82:130-40. [DOI:10.1016/j.jprot.2013.02.021]
12. Jácome AA, Sankarankutty AK, dos Santos JS. Adjuvant therapy for gastric cancer: what have we learned since INT0116? World J Gastroenterol 2015;21(13):3850-9. [DOI:10.3748/wjg.v21.i13.3850]
13. Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM, et al. Treatment of gastric cancer. World J Gastroenterol 2014;20(7):1635-49. [DOI:10.3748/wjg.v20.i7.1635]
14. Bauer K, Schroeder M, Porzsolt F, Henne-Bruns D. Comparison of international guidelines on the accompanying therapy for advanced gastric cancer: reasons for the differences. J Gastric Cancer 2015;15(1):10-8. [DOI:10.5230/jgc.2015.15.1.10]
15. Cunnane SC. Antioxidants, free radicals and PUFA. Prostaglandins Leukot Essent Fatty Acids 1994;50(6):363-4. [DOI:10.1016/0952-3278(94)90248-8]
16. Ostan R, Lanzarini C, Pini E, Scurti M, Vianello D, Bertarelli C, et al. Inflammaging and cancer: a challenge for the Mediterranean diet. Nutrients 2015;7(4):2589-621. [DOI:10.3390/nu7042589]
17. Huang C, Freter C. Lipid metabolism, apoptosis and cancer therapy. Int J Mol Sci 2015;16(1):924-49. [DOI:10.3390/ijms16010924]
18. Tiwari M, Prasad S, Tripathi A, Pandey AN, Ali I, Singh AK, et al. Apoptosis in mammalian oocytes: a review. Apoptosis 2015;20(8):1019-25. [DOI:10.1007/s10495-015-1136-y]
19. Abedi H, Aghaei M, Panjehpour M, Hajiahmadi S. Mitochondrial and caspase pathways are involved in the induction of apoptosis by IB-MECA in ovarian cancer cell lines. Tumour Biol 2014;35(11):11027-39. [DOI:10.1007/s13277-014-2396-9]
20. Zhao GY, Lin ZW, Lu CL, Gu J, Yuan YF, Xu FK, et al. USP7 overexpression predicts a poor prognosis in lung squamous cell carcinoma and large cell carcinoma. Tumour Biol 2015;36(3):1721-9. [DOI:10.1007/s13277-014-2773-4]
21. Kalkavan H, Green DR. MOMP, cell suicide as a BCL-2 family business. Cell Death Differ 2018;25(1):46-55. [DOI:10.1038/cdd.2017.179]
22. Dai J, Shen J, Pan W, Shen S, Das UN. Effects of polyunsaturated fatty acids on the growth of gastric cancer cells in vitro. Lipids Health Dis 2013;12(71):12-71. [DOI:10.1186/1476-511X-12-71]
23. Chapkin RS, Seo J, McMurray DN, Lupton JR. Mechanisms by which docosahexaenoic acid and related fatty acids reduce colon cancer risk and inflammatory disorders of the intestine. Chem Phys Lipids 2008;153(1):14-23. Epub 2008/03/04. [DOI:10.1016/j.chemphyslip.2008.02.011]
24. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37(8):911-7. [DOI:10.1139/o59-099]
25. Ariana N, Nazemi A, Nasrollahi Omran A. Using PCR to Compare the Expression of CDR1, CDR2, and MDR1 in Candida Albicans Isolates Resistant and Susceptible to Fluconazole. Med Lab . 2015;9(4):33-7. [DOI:10.18869/acadpub.mlj.9.4.33]
26. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001;25(4):402-8. [DOI:10.1006/meth.2001.1262]
27. Nourazarian SM, Ghaffarian M, Dolatkhah H. Comparison of Epicardial Adipose Tissue Fatty Acid Profile in Cardiovascular Disease Patients Diabetic and Non-Diabetic. Med Lab J 2016;10(3):13-20. [DOI:10.18869/acadpub.mlj.10.3.13]
28. Kapinova A, Kubatka P, Golubnitschaja O, Kello M, Zubor P, Solar P, et al. Dietary phytochemicals in breast cancer research: anticancer effects and potential utility for effective chemoprevention. Environ Health Prev Med 2018;23(1):36-. [DOI:10.1186/s12199-018-0724-1]
29. Langner E, Rzeski W. Dietary derived compounds in cancer chemoprevention. Contemp Oncol 2012;16(5):394-400. [DOI:10.5114/wo.2012.31767]
30. Housman G, Byler S, Heerboth S, Lapinska K, Longacre M, Snyder N, et al. Drug resistance in cancer: an overview. Cancers 2014;6(3):1769-92. [DOI:10.3390/cancers6031769]
31. Serasinghe MN, Missert DJ, Asciolla JJ, Podgrabinska S, Wieder SY, Izadmehr S, et al. Anti-apoptotic BCL-2 proteins govern cellular outcome following B-RAF(V600E) inhibition and can be targeted to reduce resistance. Oncogene. 2015;34(7):857-67. [DOI:10.1038/onc.2014.21]
32. Cunnane SC. Antioxidants, free radicals and PUFA: Prostaglandins Leukot Essent Fatty Acids 1994;50(6):363-4. [DOI:10.1016/0952-3278(94)90248-8]
33. Zhu H, Zheng Z, Zhang J, Liu X, Liu Y, Yang W, et al. Anticancer effect of 2,7-dihydroxy-3-methylanthraquinone on human gastric cancer SGC-7901 cells in vitro and in vivo. Pharm Biol 2016;54(2):285-92. [DOI:10.3109/13880209.2015.1033563]
34. Huang T-H, Wang P-W, Yang S-C, Chou W-L, Fang J-Y. Cosmetic and Therapeutic Applications of Fish Oil's Fatty Acids on the Skin. Marine drugs 2018;16(8):256. [DOI:10.3390/md16080256]
35. Hyde CA, Missailidis S. Inhibition of arachidonic acid metabolism and its implication on cell proliferation and tumour-angiogenesis. Int Immunopharmacol 2009;9(6):701-15. [DOI:10.1016/j.intimp.2009.02.003]
36. Comba A, Pasqualini ME. Primers on Molecular Pathways - Lipoxygenases: Their Role as an Oncogenic Pathway in Pancreatic Cancer. Pancreatology 2009;9(6):724-8. [DOI:10.1159/000235623]
37. Yin Y, Zhan WH, Peng JS, Zhao ZG. [Apoptosis of human gastric cancer cells induced by omega-3 polyunsaturated fatty acids]. Zhonghua Wei Chang Wai Ke Za Zhi 2007;10(6):570-3. [pubmed]
38. Jakobsen CH, Storvold GL, Bremseth H, Follestad T, Sand K, Mack M, et al. DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis. J Lipid Res 2008;49(10):2089-100. [DOI:10.1194/jlr.M700389-JLR200]
39. Slagsvold JE, Pettersen CH, Storvold GL, Follestad T, Krokan HE, Schonberg SA. DHA alters expression of target proteins of cancer therapy in chemotherapy resistant SW620 colon cancer cells. Nutr Cancer 2010;62(5):611-21. [DOI:10.1080/01635580903532366]
40. Bartram HP, Gostner A, Scheppach W, Reddy BS, Rao CV, Dusel G, et al. Effects of fish oil on rectal cell proliferation, mucosal fatty acids, and prostaglandin E2 release in healthy subjects. Gastroenterology 1993;105(5):1317-22. [DOI:10.1016/0016-5085(93)90135-Y]
41. Shirota T, Haji S, Yamasaki M, Iwasaki T, Hidaka T, Takeyama Y, et al. Apoptosis in human pancreatic cancer cells induced by eicosapentaenoic acid. Nutrition 2005;21(10):1010-7. [DOI:10.1016/j.nut.2004.12.013]
42. Dekoj T, Lee S, Desai S, Trevino J, Babcock TA, Helton WS, et al. G2/M cell-cycle arrest and apoptosis by n-3 fatty acids in a pancreatic cancer model. J Surg Res 2007;139(1):106-12. [DOI:10.1016/j.jss.2006.10.024]
43. Gago-Dominguez M, Jiang X, Castelao JE. Lipid peroxidation, oxidative stress genes and dietary factors in breast cancer protection: a hypothesis. Breast Cancer Res 2007;9(1):201. [DOI:10.1186/bcr1628]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Journal of Research in Applied and Basic Medical Sciences

Designed & Developed by : Yektaweb