Utility of newer WBC indices such as high fluorescent lymphocyte count in diagnosis and assessing the severity of dengue in the patients at a tertiary rural hospital

Swarna Latha, Pappuri; Raj B V, Swaroop

doi:10.61186/rabms.10.1.54

Volume 10, Issue 1 (1-2024) Journal of Research in Applied and Basic Medical Sciences 2024, 10(1): 54-64 | Back to browse issues page

‎ 10.61186/rabms.10.1.54

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Swarna Latha P, Raj B V S. Utility of newer WBC indices such as high fluorescent lymphocyte count in diagnosis and assessing the severity of dengue in the patients at a tertiary rural hospital. Journal of Research in Applied and Basic Medical Sciences 2024; 10 (1) :54-64
URL: http://ijrabms.umsu.ac.ir/article-1-295-en.html

Utility of newer WBC indices such as high fluorescent lymphocyte count in diagnosis and assessing the severity of dengue in the patients at a tertiary rural hospital

Pappuri Swarna Latha

, Swaroop Raj B V ^*

Associate Professor Pathology, Yenepoya Medical College, Mangaluru, Karnataka, India , swaroopraj@yenepoya.edu.in

Abstract: (1608 Views)

Background & Aims: In developing countries, lately dengue has become the most common cause of admission for fever with other infections such as malaria, enteric fever and leptospirosis on the decline. The differential diagnosis is usually sorted by serological investigation such as NS1 antigen; IgG and IgM for Dengue, though are confirmatory are expensive. The diagnosis of dengue is very important considering the sudden decline in the health of a patient due to thrombocytopenia and management of dengue which hinges on hydrating the patients and managing thrombocytopenia. The aim of this study was to study the correlation between newer parameter high fluorescent lymphocyte count (HFLC) with platelet count and dengue serology (NS1 antigen and IgM antibody).
Materials & Methods: We conducted a study at R. L. Jalappa Hospital and Research Centre, a rural tertiary and academic teaching hospital attached to Sri Devaraj Urs Medical College with a prospective study period of twelve months and between June 2019 to May 2020. A total of 386 samples were analyzed for complete blood count, Dengue serology, and HFLC count and percentage. The data was entered in excel sheet and analyzed using SPSS 22 software.
Results: The newer WBC index namely, HFLC Count correlated negatively with platelet count (r = -0.28, p < 0.05) and positively with lymphocyte percentage (r = 0.506, p < 0.05). The parameters that were statistically significant include WBC count, RBC count, hemoglobin, hematocrit, platelet count, RDW, PDW, MPV, PLCR, PCT, Neutrophil %, lymphocyte %, monocyte %, IgG%, HFLC count, HFLC % and Days in hospital including a difference in findings of these parameters between the dengue sero-positive and dengue sero-negative groups who came to the hospital with fever. Thus, highlighting that they can be used as a hint while starting prophylactic treatment until results are available when it is an emergency or when the results are delayed or in resource-limited settings in the periphery due to lack of availability of cost of serological tests.
Conclusion: In resource-limited settings, HFLC percentage and count could be used as a low cost and reliable biomarker for diagnosis and assessing the severity of dengue infection in resource-limited settings.

Keywords: Assessing the Severity, Count, Dengue, High Fluorescent Lymphocyte Diagnosis, Newer WBC Indices, Tertiary Rural Hospital

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Type of Study: orginal article | Subject: Hematology

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