Assessment of DRB1 and DQB1 genotype frequencies in type 1 diabetes: a gender-based study in Sudanese children

Omer AbdelRhman Hussein, Hiba; Salih Abdalla, Sababil; NourEldine Salih, Sakeena; A. Abdrabo, Abdelkarim; Abdelgadir Mahdi, Mohamed

doi:10.61186/rabms.10.4.420

Volume 10, Issue 4 (11-2024) Journal of Research in Applied and Basic Medical Sciences 2024, 10(4): 420-426 | Back to browse issues page

‎ 10.61186/rabms.10.4.420

Ethics code: 2022-10-12 08.52.01

Mendeley

Zotero

RefWorks

Omer AbdelRhman Hussein H, Salih Abdalla S, NourEldine Salih S, A. Abdrabo A, Abdelgadir Mahdi M. Assessment of DRB1 and DQB1 genotype frequencies in type 1 diabetes: a gender-based study in Sudanese children. Journal of Research in Applied and Basic Medical Sciences 2024; 10 (4) :420-426
URL: http://ijrabms.umsu.ac.ir/article-1-363-en.html

Assessment of DRB1 and DQB1 genotype frequencies in type 1 diabetes: a gender-based study in Sudanese children

Hiba Omer AbdelRhman Hussein

, Sababil Salih Abdalla

, Sakeena NourEldine Salih

, Abdelkarim A. Abdrabo ^*

, Mohamed Abdelgadir Mahdi

Department of Clinical Chemistry, Faculty of Applied Medical Science, Buraydah Private Colleges, Saudi Arabia , abdrabokarim@gmail.com

Abstract: (465 Views)

Background & Aims: Type 1 diabetes mellitus (T1DM) is an autoimmune condition characterized by the destruction of pancreatic β-cells. While environmental factors and autoantibodies play a role, genetic predisposition, particularly involving HLA class II alleles (DR and DQ), is significant. This study aimed to evaluate the frequency of DRB1 and DQB1 genotypes associated with T1D, with a focus on gender differences.
Materials & Methods: A total of 187 Sudanese subjects, aged 5 to 18 years, were enrolled, including 87 T1D cases and 100 non-diabetic controls. The study was conducted in diabetes hospitals in Khartoum State. HLA gene polymorphisms were assessed using the allele-specific refractory mutation system-polymerase chain reaction (ARMS-PCR) method.
Results: Genotype frequencies for C/C, G/G, and G/C were 11.8%, 66.7%, and 21.6% in females, and 10.2%, 67.3%, and 22.4% in males, respectively. Statistical analysis showed no significant gender-related differences in genotype distributions (Chi-square,
p = 0.968).
Conclusion: The study found no significant association between genotype distributions and gender in Sudanese children with T1D. This suggests that gender does not significantly influence the distribution of DRB1 and DQB1 genotypes related to T1D in the study population.

Keywords: DRB1 and DQB1 Alleles, Gender Differences, Genetic Predisposition, HLA Genotypes, Type 1 Diabetes Mellitus (T1DM)

Full-Text [PDF 290 kb] (153 Downloads)

Type of Study: orginal article | Subject: Special

References

1. Atkinson MA, Eisenbarth GS, Michels AW. Type 1 diabetes. Lancet 2014;383:69-82. [DOI:10.1016/S0140-6736(13)60591-7] [PMID]

2. American Diabetes Association. Classification and diagnosis of diabetes. Diabetes Care. 2015;38-S19. [Google Scholar]

3. Diaz-Valencia PA, Bougnères P, Valleron AJ. Global epidemiology of type 1 diabetes in young adults and adults: a systematic review. BMC Public Health 2015;15:1-5. [DOI:10.1186/s12889-015-1591-y] [PMID] [PMCID]

4. Concannon P, Rich SS, Nepom GT. Genetics of type 1A diabetes. N Engl J Med 2009;360:1646-54. [DOI:10.1056/NEJMra0808284] [PMID]

5. Redondo MJ, Jeffrey J, Fain PR, Eisenbarth GS, Orban T. Concordance for islet autoimmunity among monozygotic twins. N. Engl. J. Med 2008;359(26):2849-50. [DOI:10.1056/NEJMc0805398] [PMID]

6. Bonifacio E, Ziegler AG. Advances in the prediction and natural history of type 1 diabetes. Endocrinol. Metab. Clin. North Am 2010;39(3):513-25. [DOI:10.1016/j.ecl.2010.05.007] [PMID]

7. Noble JA, Valdes AM, Cook M, Klitz W, Thomson G, Erlich HA. The role of HLA class II genes in insulin-dependent diabetes mellitus: molecular analysis of 180 Caucasian, multiplex families. Am J Hum Genet 1996;59:1134-48. [Google Scholar]

8. Lambert AP, Gillespie KM, Thomson G, Cordell HJ, Todd JA, Gale EAM, et al. Absolute risk of childhood-onset type 1 diabetes defined by human leukocyte antigen class II genotype: a population-based study in the United Kingdom. J. Clin. Endocrinol. Metab 2004;89(8):4037-43. [DOI:10.1210/jc.2003-032084] [PMID]

9. Thomson G, Valdes AM, Noble JA, Kockum I, Grote MN, Najman J, et al. Relative predispositional effects of HLA class II DRB1-DQB1 haplotypes and genotypes on type 1 diabetes: a meta-analysis. Tissue Antigens 2007;70(2):110-27. [DOI:10.1111/j.1399-0039.2007.00867.x] [PMID]

10. Ziegler AG, Hummel M, Schenker M, Bonifacio E. Autoantibody appearance and risk for development of childhood diabetes in offspring of parents with type 1 diabetes: the 2-year analysis of the German BABYDIAB Study. Diabetes 1999;48(3):460-8. [DOI:10.2337/diabetes.48.3.460] [PMID]

11. Svensson J, Carstensen B, Mortensen HB, Borch-Johnsen K, Danish Study Group of Diabetes in Childhood. Gender-associated differences in Type 1 diabetes risk factors? Diabetologia 2003;46(3):442-3. [DOI:10.1007/s00125-003-1049-0] [PMID]

12. Arhire AI, Ioacara S, Papuc T, Chiper MS, Dutescu IM, Moise A, Badea IR, Florea S, Vlad A, Fica S. Association of HLA haplotypes with autoimmune pathogenesis in newly diagnosed type 1 Romanian diabetic children: A pilot, single-center cross-sectional study. Life 2024;20;14(6):781 [DOI:10.3390/life14060781] [PMID] [PMCID]

13. Hunt SE, Moore B, Amode RM, Armean IM, Lemos D, Mushtaq A, et al. Annotating and prioritizing genomic variants using the Ensembl Variant Effect Predictor-A tutorial. Hum. Mutat 2022a;43(8):986-97 [DOI:10.1002/humu.24298] [PMID] [PMCID]

14. Haris B, Ahmed I, Syed N, Almabrazi H, Saraswathi S, Al-Khawaga S, et al. Clinical features, epidemiology, autoantibody status, HLA haplotypes and genetic mechanisms of type 1 diabetes mellitus among children in Qatar. Sci. Rep 2021;11(1):18887. [DOI:10.1038/s41598-021-98460-4] [PMID] [PMCID]

15. Redondo MJ, Steck AK, Pugliese A. Genetics of type 1 diabetes. Pediatr. Diabetes 2018;19(3):346-53. [DOI:10.1111/pedi.12597] [PMID] [PMCID]

16. Stene LC, Norris JM, Rewers MJ. Risk Factors for Type 1 Diabetes. In: Lawrence JM, Casagrande SS, Herman WH, Wexler DJ, Cefalu WT, editors. Diabetes in America [Internet]. Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); 2023 [cited 2024 Nov 20]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK597412/ [Google Scholar]

17. Morran MP, Vonberg A, Khadra A, Pietropaolo M. Immunogenetics of type 1 diabetes mellitus. Molecular aspects of medicine 2015;42:42-60. [DOI:10.1016/j.mam.2014.12.004] [PMID] [PMCID]

18. Tatti P, Pavandeep S. Gender difference in type 1 diabetes: An underevaluated dimension of the disease. Diabetology 2022;3(2):364-8. [DOI:10.3390/diabetology3020027]