A study on optimal biopsy site in patients presenting with dyspepsia for Helicobacter pylori by using the Sydney System of grading

Gopidesi, Divya Tejaswi; Recharla, Madhuri; Vallapureddy, Thejaswini; Jonnadula, Pratima; K, Durga; Nandam, Mohan Rao; Begam, Vaheda

doi:10.61186/rabms.11.2.193

Volume 11, Issue 2 (4-2025) Journal of Research in Applied and Basic Medical Sciences 2025, 11(2): 193-201 | Back to browse issues page

‎ 10.61186/rabms.11.2.193

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Gopidesi D T, Recharla M, Vallapureddy T, Jonnadula P, K D, Nandam M R et al . A study on optimal biopsy site in patients presenting with dyspepsia for Helicobacter pylori by using the Sydney System of grading. Journal of Research in Applied and Basic Medical Sciences 2025; 11 (2) :193-201
URL: http://ijrabms.umsu.ac.ir/article-1-392-en.html

A study on optimal biopsy site in patients presenting with dyspepsia for Helicobacter pylori by using the Sydney System of grading

Divya Tejaswi Gopidesi ^*

, Madhuri Recharla

, Thejaswini Vallapureddy

, Pratima Jonnadula

, Durga K

, Mohan Rao Nandam

, Vaheda Begam

Assistant Professor, Department of Pathology, Narayana Medical College and Hospital, Nellore, Andhra Pradesh, India , divyateja57@gmail.com

Abstract: (223 Views)

Background & Aims: Helicobacter pylori (H. pylori) infection, a common organic cause of dyspepsia, often lacks macroscopic mucosal lesions, necessitating distinction from functional dyspepsia. This study assessed H. pylori prevalence, optimal biopsy sites, histopathological changes, and infection density in dyspeptic patients.
Materials & Methods: Gastric mucosal biopsies from 100 dyspeptic patients were formalin-fixed, paraffin-embedded, and sectioned (5μm). Hematoxylin and eosin (H&E) and Giemsa stains were used for histopathological evaluation, with Giemsa specifically applied for H. pylori grading.
Results: Patients (mean age: 44.37 years; range: 11–80) showed peak H. pylori prevalence in the fourth (28%, n=28) and fifth (29%, n=29) decades, with a male-to-female ratio of 1.26:1. Common symptoms included nausea (76%, n=76), epigastric discomfort, abdominal pain, and bloating. Chronic gastritis was identified in 89% (n=89), with 82% (n=82) testing H. pylori-positive, all exhibiting chronic active gastritis. The pyloric antrum was the predominant colonization site (86.58%, n=71/82), followed by the fundus (84.14%, n=69/82) and body (74.39%, n=61/82). Per the Sydney System, inflammation severity was mild (42.68%, n=35), moderate (48.78%, n=40), or severe (8.54%, n=7). H. pylori density was graded as mild (36.59%, n=30), moderate (54.88%, n=45), or dense (8.53%, n=7).
Conclusion: Early H. pylori detection and eradication alleviate symptoms and prevent complications. Giemsa stain proved optimal for H. pylori identification due to its cost-effectiveness and rapidity. The pyloric antrum, followed by the fundus and body, is the primary biopsy site for diagnosing H. pylori-associated gastritis. These findings emphasize targeted biopsy protocols and efficient diagnostic methods in managing dyspepsia.

Keywords: Dyspepsia, Gastritis, Haematoxylin and Eosin, H.pylori, Sydney System

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Type of Study: orginal article | Subject: General

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