Volume 11, Issue 3 (8-2025)
Journal of Research in Applied and Basic Medical Sciences 2025, 11(3): 271-279 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Adebajo O A, Gbotolorun C S, Oremosu A, Adebajo P K, Ojo J H, Udom Akpan U, et al . The Histomorphometric Alterations in Male Sprague-Dawley Rats Following Exposure to Dextromethorphan and Antioxidants Administration. Journal of Research in Applied and Basic Medical Sciences 2025; 11 (3) :271-279
URL: http://ijrabms.umsu.ac.ir/article-1-429-en.html
URL: http://ijrabms.umsu.ac.ir/article-1-429-en.html
Oluwaseye Adesina Adebajo * 
, Chinwe Stella Gbotolorun , Ayodele Oremosu , Perpetual Kuburat Adebajo , Joshua Honor Ojo , Utom-Obong Udom Akpan , Zainab Adebusola Sogbesan , Bassey Helen Akpan , Gloria Inioluwa Okegbemi , Jesulayomi Oluwayemisi Oladipo , Oyindamola Dorcas Akinwola
, Chinwe Stella Gbotolorun , Ayodele Oremosu , Perpetual Kuburat Adebajo , Joshua Honor Ojo , Utom-Obong Udom Akpan , Zainab Adebusola Sogbesan , Bassey Helen Akpan , Gloria Inioluwa Okegbemi , Jesulayomi Oluwayemisi Oladipo , Oyindamola Dorcas Akinwola
Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine of the University of Lagos, Lagos, Nigeria & Anatomy Programme, College of Health Sciences, Bowen University, Iwo campus, Osun State, Nigeria , oluwaseye.adebajo@bowen.edu.ng
Abstract: (36 Views)
Background Morphometric evaluation of seminiferous tubules and epididymis is a common parameter used to assess male fertility. This study aimed to evaluate the morphological changes in male rats following exposure to dextromethorphan.
Methods A total of 80 male rats (150 ± 30 g) were divided into four groups (N = 20; A-D) for the study. Group A received distilled water; group B received 20 mg/kg, group C received 40 mg/kg, and group D received 80 mg/kg of DM for a duration of four weeks. At the end of the treatment period, five rats were selected from each group and the following histomorphometric parameters were analyzed: diameter and height of the seminiferous tubule and epididymes, volume of testes, and the number of spermatogonia, spermatocytes, and spermatids within the seminiferous tubules. The remaining 15 rats were divided into three groups (N = 5; E-G). They received Rutin (25 mg/kg), Quercetin (50 mg/kg), and DW, respectively, for four weeks to ascertain the recovery rate.
Results All histomorphometric parameters decreased compared to the control group; however, when comparing the recovery-alone group to the treatment groups, a slight increase was observed. The morphometric measurements showed an increase when Rutin and Quercetin were compared to both the treatment and recovery-alone groups. The comparison between Rutin and Quercetin showed no substantial difference in their effects.
Conclusion Dextromethorphan has showed to have deleterious effect on the epididymal and testicular morphometry and this could culminate into infertility in males, however, these damages were significantly improved upon the administration of Rutin and Quercetin as antioxidants.
Methods A total of 80 male rats (150 ± 30 g) were divided into four groups (N = 20; A-D) for the study. Group A received distilled water; group B received 20 mg/kg, group C received 40 mg/kg, and group D received 80 mg/kg of DM for a duration of four weeks. At the end of the treatment period, five rats were selected from each group and the following histomorphometric parameters were analyzed: diameter and height of the seminiferous tubule and epididymes, volume of testes, and the number of spermatogonia, spermatocytes, and spermatids within the seminiferous tubules. The remaining 15 rats were divided into three groups (N = 5; E-G). They received Rutin (25 mg/kg), Quercetin (50 mg/kg), and DW, respectively, for four weeks to ascertain the recovery rate.
Results All histomorphometric parameters decreased compared to the control group; however, when comparing the recovery-alone group to the treatment groups, a slight increase was observed. The morphometric measurements showed an increase when Rutin and Quercetin were compared to both the treatment and recovery-alone groups. The comparison between Rutin and Quercetin showed no substantial difference in their effects.
Conclusion Dextromethorphan has showed to have deleterious effect on the epididymal and testicular morphometry and this could culminate into infertility in males, however, these damages were significantly improved upon the administration of Rutin and Quercetin as antioxidants.
Type of Study: orginal article |
Subject:
Other
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Contact Information
Address: Urmia University of Medical Sciences, Jahad Ave., Urmia, I.R. Iran. Post code: 57147-83734. P.O. Box. 1138
Email: ijrabms
gmail.com
rabmsumsu.ac.ir
Tel / Fax: +984431937235/+984432240642
