Volume 11, Issue 4 (11-2025)
Journal of Research in Applied and Basic Medical Sciences 2025, 11(4): 370-376 |
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Putti P, Rohith K, bhaskar Potuganti V, Surya Prabha M L, Aggarwal J, Mohiuddin M, et al . Identification of CTLA-4 +49 A/G (Rs231775) Polymorphism in Diabetic Retinopathy and its Allelic Association with Cardiovascular Disease Risk Factors. Journal of Research in Applied and Basic Medical Sciences 2025; 11 (4) :370-376
URL: http://ijrabms.umsu.ac.ir/article-1-440-en.html
URL: http://ijrabms.umsu.ac.ir/article-1-440-en.html
Praneeth Putti * 
, Karnati Rohith , Vijaya Bhaskar Potuganti , M Lakshmi Surya Prabha , Juhi Aggarwal , Meraj Mohiuddin , Shabana Shireen , Babulal S , Swarna Deepak Kuragayala , Mahaboob Vali Shaik
, Karnati Rohith , Vijaya Bhaskar Potuganti , M Lakshmi Surya Prabha , Juhi Aggarwal , Meraj Mohiuddin , Shabana Shireen , Babulal S , Swarna Deepak Kuragayala , Mahaboob Vali Shaik
Department of Ophthalmology, Mallareddy Institute of Medical Sciences, GHMC Quthbullapur, Jeedimetla, Hyderabad, Telangana, India , praneeth84@gmail.com
Abstract: (74 Views)
Background & Aim: The Study investigates the association between the CTLA-4 +49 A/G (rs231775) polymorphism and cardiovascular disease risk factors in patients with diabetic retinopathy.
Methods & Materials: Fifty cases with diabetic retinopathy were compared to fifty healthy controls. Blood samples were subjected to ARMS-PCR with primers specific to the CTLA-4 +49 A/G (rs231775) gene.
Results: Hypertension was identified as a co-factor for diabetic retinopathy, with an odds ratio of 1.32 (95% CI: 0.45-4.85). Furthermore, the A allele was more frequently observed in patients with a history of myocardial infarction than the G allele, with counts of 25 versus 10 and 11 versus 4 (p = 0.455). The A allele was also more prevalent in the hyperlipidemia group than the G allele, with frequencies of 34 versus 1 and 9 versus 6 (p = 0.255). Hyperlipidemia was recognized as a co-factor for diabetic retinopathy, with an odds ratio of 2.25 (95% CI: 0.42-12.65). The A allele showed an odds ratio of 3.85 (95% CI: 1.95-7.99), indicating an increased risk of developing diabetic retinopathy. The AG genotype was significantly more common in the diabetic retinopathy group compared to the control group (31 versus 11) (p = 0.003), and this genotype was associated with a higher risk for diabetic retinopathy (odds ratio: 12.2, 95% CI: 4.56-41.85). The AA genotype was more frequently observed in the diabetic retinopathy group than the control group (15 versus 22) (p = 0.312) (odds ratio: 3.2, 95% CI: 0.598-7).
Conclusion: The AG genotype and the A allele were more prevalent in patients with diabetic retinopathy, suggesting that they may be risk factors. The AA genotype, the AG genotype, and the G allele exhibited significant associations with diabetic retinopathy.
Methods & Materials: Fifty cases with diabetic retinopathy were compared to fifty healthy controls. Blood samples were subjected to ARMS-PCR with primers specific to the CTLA-4 +49 A/G (rs231775) gene.
Results: Hypertension was identified as a co-factor for diabetic retinopathy, with an odds ratio of 1.32 (95% CI: 0.45-4.85). Furthermore, the A allele was more frequently observed in patients with a history of myocardial infarction than the G allele, with counts of 25 versus 10 and 11 versus 4 (p = 0.455). The A allele was also more prevalent in the hyperlipidemia group than the G allele, with frequencies of 34 versus 1 and 9 versus 6 (p = 0.255). Hyperlipidemia was recognized as a co-factor for diabetic retinopathy, with an odds ratio of 2.25 (95% CI: 0.42-12.65). The A allele showed an odds ratio of 3.85 (95% CI: 1.95-7.99), indicating an increased risk of developing diabetic retinopathy. The AG genotype was significantly more common in the diabetic retinopathy group compared to the control group (31 versus 11) (p = 0.003), and this genotype was associated with a higher risk for diabetic retinopathy (odds ratio: 12.2, 95% CI: 4.56-41.85). The AA genotype was more frequently observed in the diabetic retinopathy group than the control group (15 versus 22) (p = 0.312) (odds ratio: 3.2, 95% CI: 0.598-7).
Conclusion: The AG genotype and the A allele were more prevalent in patients with diabetic retinopathy, suggesting that they may be risk factors. The AA genotype, the AG genotype, and the G allele exhibited significant associations with diabetic retinopathy.
Type of Study: orginal article |
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