Comparative Phytochemical, Safety, and Analgesic Evaluation of Selected
African Medicinal Plants in Swiss Albino Mice

Tahir, Albashir; Abba Usman, Musab; Khaleel Muazu, Ibrahim; Muhammad Lawan, Muhammad; Ahmad Muhammad, Muslim; Ahmad, Sanusi; Ahmad, Saddam

doi:https://doi.org/10.61882/rabms.11.4.390

Volume 11, Issue 4 (11-2025) Journal of Research in Applied and Basic Medical Sciences 2025, 11(4): 390-397 | Back to browse issues page

‎ https://doi.org/10.61882/rabms.11.4.390

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Tahir A, Abba Usman M, Khaleel Muazu I, Muhammad Lawan M, Ahmad Muhammad M, Ahmad S et al . Comparative Phytochemical, Safety, and Analgesic Evaluation of Selected African Medicinal Plants in Swiss Albino Mice. Journal of Research in Applied and Basic Medical Sciences 2025; 11 (4) :390-397
URL: http://ijrabms.umsu.ac.ir/article-1-455-en.html

Comparative Phytochemical, Safety, and Analgesic Evaluation of Selected African Medicinal Plants in Swiss Albino Mice

Albashir Tahir ^*

, Musab Abba Usman

, Ibrahim Khaleel Muazu

, Muhammad Muhammad Lawan

, Muslim Ahmad Muhammad

, Sanusi Ahmad

, Saddam Ahmad

Department of Pharmacology, Faculty of Basic Medical Sciences, Sa'adu Zungur University, Bauchi State , albashirtahir@sazu.edu.ng

Abstract: (124 Views)

Background Moringa oleifera, Anogeissus leiocarpus, and Commiphora africana are medicinal plants traditionally
used for managing pain and inflammation. Despite documented pharmacological properties, the comparative evaluation
of their analgesic activities using standardized peripheral and central pain models is limited.This study evaluated and
compared the analgesic activities, phytochemical profiles, and acute toxicity of methanol extracts of M. oleifera seeds,
A. leiocarpus leaves, and C. africana leaves in Swiss albino mice.
Methods Plant materials were extracted with methanol and subjected to qualitative phytochemical screening. Acute
oral toxicity was evaluated using Lorke’s method. Analgesic effects were evaluated using the acetic acid-induced
writhing assay and the hot plate method at doses of 150, 300, and 600 mg/kg (i.p.). Piroxicam (10 mg/kg) and
pentazocine (1 mg/kg) served as standard drugs.
Results All extracts contained alkaloids, flavonoids, saponins, and tannins, with terpenoids detected only in M.
oleifera and steroids only in A. leiocarpus. LD₅₀ values were > 5000 mg/kg for M. oleifera and A. leiocarpus, and
1264.9 mg/kg for C. africana. In the acetic acid-induced writhing assay, a significant, dose-dependent reduction
in abdominal constrictions was observed in the extract-treated group. Similarly, in the hot plate test, all extracts
significantly prolonged reaction latencies, with C. africana at 600 mg/kg approaching the activity of pentazocine.
Conclusion Methanol extracts of M. oleifera, A. leiocarpus, and C. africana possess significant peripheral and central
analgesic activities, likely mediated by their bioactive phytoconstituents. The study supports their ethnomedicinal use
in pain management and highlights C. africana’s potent central analgesic activity but narrower safety margin. Further
bioassay-guided and mechanistic studies are warranted.

Keywords: Acute toxicity, Analgesic activity, Anogeissus leiocarpus, Commiphora africana, Moringa oleifera, Phytochemicals

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Type of Study: orginal article | Subject: Other

References

1. Liu S, Kelliher L. Physiology of pain-a narrative review on the pain pathway and its application in the pain management. Digestive Medicine Research. 2022;5. doi: 10.21037/dmr-21-100. [DOI:10.21037/dmr-21-100]

2. Cao B, Xu Q, Shi Y, Zhao R, Li H, Zheng J, et al. Pathology of pain and its implications for therapeutic interventions. Signal Transduct Target Ther. 2024;9(1):155. doi: 10.1038/s41392-024-01845-w. [DOI:10.1038/s41392-024-01845-w] [PMID] [PMCID]

3. Adekunle YA, Samuel BB, Nahar L, Fatokun AA, Sarker SD. Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae): A review of the traditional uses, phytochemistry and pharmacology of African birch. Fitoterapia. 2024;176:105979. doi: 10.1016/j.fitote.2024.105979 [DOI:10.1016/j.fitote.2024.105979] [PMID]

4. Meireles D, Gomes J, Lopes L, Hinzmann M, Machado J. A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Advances in Traditional Medicine. 2020;20(4):495-515. doi: 10.1007/s13596-020-00468-0. [DOI:10.1007/s13596-020-00468-0] [PMCID]

5. Yang Y, Sun X, Peng C, Wei J, Yang X. The Genus Commiphora: An Overview of Its Traditional Uses, Phytochemistry, Pharmacology, and Quality Control. Pharmaceuticals (Basel). 2024;17(11):1524. doi: org/10.3390/ph17111524. [DOI:10.3390/ph17111524] [PMID] []

6. Dauda A, Usman AM, Tahir A, Muhammad AM, Yunusa S, Kura AU. Pharmacological Evaluation of Moringa oleifera Seeds Extract for Anti-Inflammatory Effects in a Mouse Model. Adv Pharmacol Pharm. 2024;12(1):66 â-70. doi: org/10.13189/app.2024.120108. [DOI:10.13189/app.2024.120108]

7. Soto JA, Gómez AC, Vásquez M, Barreto AN, Molina KS, Zuniga-Gonzalez CA. Biological properties of Moringa oleifera: A systematic review of the last decade. F1000Res. 2025;13:1390. doi: 10.12688/f1000research.157194.2. [DOI:10.12688/f1000research.157194.2] [PMID] [PMCID]

8. Ahmed IM, Gadir SA, Elgilany EE, Abdallah TM. Commiphora africana Resin Phytochemical Analysis & Some Biological Aspects. European Journal of Medicinal Plants. 2016;13(3). doi: 10.9734/EJMP/2016/22531. [DOI:10.9734/EJMP/2016/22531]

9. Rengasamy KRR, Mahomoodally MF, Joaheer T, Zhang Y. A Systematic Review of Traditionally Used Herbs and Animal-Derived Products as Potential Analgesics. Curr Neuropharmacol. 2021;19(4):553-88. doi: 10.2174/1570159X18666200808151522. [DOI:10.2174/1570159X18666200808151522] [PMID] [PMCID]

10. Patel M, Wahezi S, Mavrocordatos P, Abd-Elsayed A. The Effects and Mechanisms of Phytochemicals on Pain Management and Analgesic. Nutrients. 2025;17(4):633. doi: 10.3390/nu17040633. [DOI:10.3390/nu17040633] [PMID] [PMCID]

11. R Silva-Correa C. Potential Activity of Medicinal Plants as Pain Modulators: A Review. Pharmacognosy Journal. 2021 Jan 8;13(1):248-63. doi:10.5530/pj.2021.13.35 [DOI:10.5530/pj.2021.13.35]

12. Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54(4):275-87. doi: 10.1007/BF01234480. [DOI:10.1007/BF01234480] [PMID]

13. Harborne AJ. Phytochemical methods: a guide to modern techniques of plant analysis. springer science & business media.1998. [Google Books]

14. Trease GE, Evans WC. Pharmacognosy. 9th edn. London: Saunders Publishers; 2002. 553-557 p. [URL]

15. Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Federation Proceedings. 1959;18(1):412-7. [URL]

16. OECD. Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure [Internet]. OECD; 2022 [cited 2026 Feb 20]. (OECD Guidelines for the Testing of Chemicals, Section 4). doi: 10.1787/9789264071049-en [DOI:10.1787/9789264071049-en]

17. Cho IH, Lee MJ, Jang M, Gwak NG, Lee KY, Jung HS. Minocycline Markedly Reduces Acute Visceral Nociception via Inhibiting Neuronal ERK Phosphorylation. Mol Pain. 2012 ; 8-13. doi: 10.1186/1744-8069-8-13. [DOI:10.1186/1744-8069-8-13] [PMID] [PMCID]

18. Lanhers MC, Fleurentin J, Mortier F, Vinche A, Younos C. Anti-Inflammatory and Analgesic Effects of an Aqueous Extract of Harpagophytum procumbens. Planta Med. 1992;58 (2):117-23. doi: 10.1055/s-2006-961411. [DOI:10.1055/s-2006-961411] [PMID]

19. Santenna C, Kumar S, Balakrishnan S, Jhaj R, Ahmed SN. A comparative experimental study of analgesic activity of a novel non-steroidal anti-inflammatory molecule - zaltoprofen, and a standard drug - piroxicam, using murine models. J Exp Pharmacol. 2019 ;11:85-91. doi: 10.2147/JEP.S212988. [DOI:10.2147/JEP.S212988] [PMID] [PMCID]

20. Ide S, Minami M, Uhl GR, Satoh M, Sora I, Ikeda K. (-)-Pentazocine induces visceral chemical antinociception, but not thermal, mechanical, or somatic chemical antinociception, in μ-opioid receptor knockout mice. Mol Pain. 2011;7:23. doi: 10.1186/1744-8069-7-23. [DOI:10.1186/1744-8069-7-23] [PMID] [PMCID]

21. Nisar A, Jagtap S, Vyavahare S, Deshpande M, Harsulkar A, Ranjekar P, et al. Phytochemicals in the treatment of inflammation-associated diseases: the journey from preclinical trials to clinical practice. Front Pharmacol. 2023;14:1177050. doi: 10.3389/fphar.2023.1177050. [DOI:10.3389/fphar.2023.1177050] [PMID] [PMCID]

22. Omeje E, Osadebe P, Obonga W, Ugwuoke C. Phytochemical and Pharmacological Basis for the Ethnomedicinal Use of Root Extracts from Anogeissus leiocarpus as an Antidiabetic in Eastern Nigeria. British Journal of Pharmaceutical Research. 2016;9(4):1-8. doi: 10.9734/BJPR/2016/22289. [DOI:10.9734/BJPR/2016/22289]

23. Jomova K, Alomar SY, Valko R, Liska J, Nepovimova E, Kuca K, Valko M, et al. Flavonoids and their role in oxidative stress, inflammation, and human diseases. Chemico-Biological Interactions. 2025;413:111489. doi: 10.1016/j.cbi.2025.111489. [DOI:10.1016/j.cbi.2025.111489] [PMID]

24. Wijesekara T, Luo J, Xu B. Critical review on anti-inflammation effects of saponins and their molecular mechanisms. Phytother Res. 2024;38 (4):2007-2022. doi: 10.1002/ptr.8164. [DOI:10.1002/ptr.8164] [PMID]

25. Idakwoji PA, Atanu FO, Nweje-Anyalowu PC, Momoh TB, Oniwon WO, Elazab ST, et al. Pharmacological studies of anti-inflammatory, anti-nociceptive and anti-pyretic compounds found in chromatographic fractions of Anogeissus leiocarpa (DC). Guill. & Perr. leaves. J Pharm Pharmacogn Res. 2022;10(3):459-68. doi: 10.56499/jppres21.1265_10.3.459. [DOI:10.56499/jppres21.1265_10.3.459]

26. Alemu A, Tamiru W, Nedi T, Shibeshi W. Analgesic and Anti-Inflammatory Effects of 80% Methanol Extract of Leonotis ocymifolia (Burm.f.) Iwarsson Leaves in Rodent Models. Evid Based Complement Alternat Med. 2018;1614793. doi: 10.1155/2018/1614793. [DOI:10.1155/2018/1614793] [PMID] [PMCID]

27. Gonfa YH, Tessema FB, Bachheti A, Rai N, Tadesse MG, Singab AN, et al. Anti-inflammatory activity of phytochemicals from medicinal plants and their nanoparticles: A review. Current Research in Biotechnology. 2023;6:100152. doi: 10.1016/j.crbiot.2023.100152. [DOI:10.1016/j.crbiot.2023.100152]

28. Santiago LÂ, Neto RN, Santos Ataíde AC, Fonseca DC, Soares EF, de Sá Sousa JC, et al. Flavonoids, alkaloids and saponins: are these plant-derived compounds an alternative to the treatment of rheumatoid arthritis? A literature review. Clinical Phytoscience. 2021;7(1):58. doi: 10.1186/s40816-021-00291-3. [DOI:10.1186/s40816-021-00291-3]