Volume 9, Issue 3 (7-2023)
Journal of Research in Applied and Basic Medical Sciences 2023, 9(3): 130-137 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Usunobun U, Okpiabhele A. Telfairia occidentalis Hook f. mitigates Carbon Tetrachloride induced Nephrotoxicity in Rat. Journal of Research in Applied and Basic Medical Sciences 2023; 9 (3) :130-137
URL: http://ijrabms.umsu.ac.ir/article-1-246-en.html
URL: http://ijrabms.umsu.ac.ir/article-1-246-en.html
Department of Biochemistry, Faculty of Basic Medical Sciences, Edo State University Uzairue, Edo State, Nigeria , usunobun.usunomena@edouniversity.edu.ng
Abstract: (814 Views)
Background & Aims: Telfairia occidentalis Hook f. is consumed in different parts of the Niger-Delta region of Nigeria due to its high nutritional and medicinal benefits. This study focused on the restorative potentials of Telfaria occidentalis aqueous leaf extract on carbon tetrachloride (CCl4)-induced renal toxicity in wistar rats.
Materials & Methods: In this experimental study, five experimental groups of rats were used. One group received distilled water and serve as normal control. Second group received carbon tetrachloride (CCl4) alone for four days. Third and fourth groups received CCl4 for four days prior to treatment with 200 mg/kg and 400 mg/kg T. occidentalis aqueous extract for six days, respectively. The last group received CCl4 for four days prior to treatment with Silymarin (100 mg/kg) for six days. With exception of normal control rats, all rats received a mixture of freshly prepared CCl4 in olive oil (1 ml/kg, 1:1 intraperitoneally) for 4 days. Activities of renal markers and lipid profile molecules in serum and histopathogical analysis were assessed. Differences between means of groups were determined by One-way ANOVA using SPSS v.20. The mean differences were compared with the Duncan multiple range test. A probability level of less than 5% (P˂0.05) was considered significant.
Results: Results revealed that CCl4 toxicity caused a significant increase (P < 0.05) in the level of serum kidney function markers (Creatinine and Urea) and in lipid profile molecules (Total Cholesterol and Triglycerides) whereas T. occidentalis administration showed a dose-dependent nephro-protection as it significantly mitigated the effects of CCl4 on the kidney function markers and lipid profile molecules assessed. The observed CCl4 toxicity and renal protection by T. occidentalis were corroborated by the results of histopathological analysis.
Conclusion: The results showed that T. occidentalis aqueous leaf extract mitigated the exacerbated effect of CCl4 on renal functions which can be attributed to its bioactive agents.
Materials & Methods: In this experimental study, five experimental groups of rats were used. One group received distilled water and serve as normal control. Second group received carbon tetrachloride (CCl4) alone for four days. Third and fourth groups received CCl4 for four days prior to treatment with 200 mg/kg and 400 mg/kg T. occidentalis aqueous extract for six days, respectively. The last group received CCl4 for four days prior to treatment with Silymarin (100 mg/kg) for six days. With exception of normal control rats, all rats received a mixture of freshly prepared CCl4 in olive oil (1 ml/kg, 1:1 intraperitoneally) for 4 days. Activities of renal markers and lipid profile molecules in serum and histopathogical analysis were assessed. Differences between means of groups were determined by One-way ANOVA using SPSS v.20. The mean differences were compared with the Duncan multiple range test. A probability level of less than 5% (P˂0.05) was considered significant.
Results: Results revealed that CCl4 toxicity caused a significant increase (P < 0.05) in the level of serum kidney function markers (Creatinine and Urea) and in lipid profile molecules (Total Cholesterol and Triglycerides) whereas T. occidentalis administration showed a dose-dependent nephro-protection as it significantly mitigated the effects of CCl4 on the kidney function markers and lipid profile molecules assessed. The observed CCl4 toxicity and renal protection by T. occidentalis were corroborated by the results of histopathological analysis.
Conclusion: The results showed that T. occidentalis aqueous leaf extract mitigated the exacerbated effect of CCl4 on renal functions which can be attributed to its bioactive agents.
Type of Study: orginal article |
Subject:
General
References
1. Hazafa A, Rehman KU, Jahan N, Jabeen Z. The role of polyphenol (flavonoids) compounds in the treatment of cancer cells. Nutr Cancer 2020;72(3):386-97. [DOI:10.1080/01635581.2019.1637006] [PMID]
2. Chakraborty S, Majumdar S. Natural products for the treatment of pain: Chemistry and pharmacology of salvinorin A, mitragynine, and collybolide. Biochemistry 2021;60(18):1381-400. [DOI:10.1021/acs.biochem.0c00629] [PMID] []
3. James SA, Omwirhiren RE, Joshua IA, Dutse I. Anti-diabetic properties and phytochemical studies of ethanolic leaf extracts of Murraya koenigii and Telfairia occidentalis on alloxan-induced diabetic albino rats. Ornament 2016;49. [DOI:10.53858/arocnpr01015260]
4. Okoli BE, Mgbeogu C. Fluted pumpkin, Telfairia occidentalis: West African vegetable crop. Econ Bot 1983,37:145-9.
https://doi.org/10.1007/BF02858775 [DOI:10.1007/bf02858775]
5. Ehiagbonare JE. Conservation studies on Telfairia occidentalis Hook. f. A. indigenous plant used in enthnomedicinal treatment of anemia in Nigeria. Afr J Agric Res 2008;3:74-7. [Google Scholar]
6. Oboh G. Hepatoprotective Property of Ethanolic and Aqueous Extracts of Fluted Pumpkin (Telfairia occidentalis) leaves against Garlic-induced Oxidative Stress. J Med Food 2005;8(4):560-563 [DOI:10.1089/jmf.2005.8.560] [PMID]
7. AS, Eseyin OA, Etim EI, Okokon JE, Anah VU, Attih EE, et al. Telfairia occidentalis potentiates the antiplasmodial activity of artemisinins and amodiaquine combination therapy. Antiinfect Agents 2020;18(2):152-9. [DOI:10.2174/2211352517666190206160812]
8. Kayoed AA, Kayode OT. Some Medicinal Values of Telfairia occidentalis: A review. Am J Biochem Mol Biol 2011;1(1):30-38. [DOI:10.3923/ajbmb.2011.30.38]
9. Usunobun U, Egharebva E. Phytochemical analysis, proximate and mineral composition and in vitro antioxidant activities in Telfairia occidentalis aqueous leaf extract. J Basic App Sci 2014;1(1):74-87. [Google Scholar]
10. Aderibigbe AO, Lawal BAS, Oluwagbemi JO. The anti-hyperglyceamic effect of Telfairia occidentalis in mice. African J Med Sci 1999;28:171-5. [PMID]
11. Nwozo SO, Adaramoye OA, Ajaiyeoba EO. Anti-diabetic and hypo lipidaemic studies of Telfairia occidentalis on alloxan induced diabetic rabbits. Nig J Nat Prod Med 2004;8:45-7. [DOI:10.4314/njnpm.v8i1.11814]
12. Okokon JE, Ekpo AJ, Eseyin OA. Antiplasmodial activity of ethanolic root extract of Telfiaria occidentalis. Res J Parasitol 2007;2:94-8. [DOI:10.3923/jp.2007.94.98]
13. Adaramoye OA, Achem J, Akintayo OO, Fafunso MA. Hypolipidaemic effect of Telfairia occidentalis [fluted pumpkin] in rats fed a cholesterol-rich diet. J Med Food 2007;10:316-30 [DOI:10.1089/jmf.2006.213] [PMID]
14. Oboh G, Akindahunsi AA. Change in the ascorbic acid total phenol and antioxidant activity of sun-dried commonly consumedgreen leafy vegetables in Nigeria. Nutr Health 2004;18:29-36. [DOI:10.1177/026010600401800103] [PMID]
15. Kayode OT, Kayode AA, Adetola AA. Therapeutic effect of Telfairia occidentalis on protein energy malnutrition- Induced liver damage. Res J Med Plant 2009;3:80-92. [DOI:10.3923/rjmp.2009.80.92]
16. Kayode AA, Kayode OT, Adetola AA. Telfairia occidentalis ameliorates oxidative brain damage in malnourished rat. Int J Biol Chem 2010;4:10-8. [DOI:10.3923/ijbc.2010.10.18]
17. APA. Guidelines for Ethical Conduct in the Care and Use of Non-Human Animals in Research. American Psychological Association (APA), Washington DC, USA. 2012.
18. Saalu LC, Kpela T, Benebo AS, Oyewopo AO, Anifowope EO, Oguntola A, The dose-dependent testiculoprotective and testiculotoxic potentials of Telfairia occidentalis Hook f. leaves extract in rat. Int J Appl Res Nat Prod 2010;3:27-38. [Google Scholar]
19. Akang N, Oremosu AA, Osinubi AA, Dosumu OO, Kusemiju TO, Adelakun SA, Umaru ML. Histomorphometric studies of the effects of Telfairia occidentalis onalcohol-induced gonado-toxicity in male rats. Toxicology Reports 2015;2:968-975. [DOI:10.1016/j.toxrep.2015.06.009] [PMID] []
20. Fawcett JK, Scott JE. A rapid precise method for the determination of urea. J Clin Path 1960;13(2):156-9. [DOI:10.1136/jcp.13.2.156] [PMID] []
21. Searcy PI, Bergquist LM. A new color reaction for the quantatiation of serum cholesterol. Clin Chem Acta 1960;5:192-9. [DOI:10.1016/0009-8981(60)90035-8] [PMID]
22. Tiez NW. Clinical guide to laboratory tests. 2nd Edn., W.B. Saunders company, Philadelphia, USA, 1990;pp: 554. [URL]
23. Mitchell HR, Kline W. Core curriculum in nephrology, renal function testing. Am J Kidney Dis 2006;47:174-83. [DOI:10.1053/j.ajkd.2005.08.038] [PMID]
24. Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AAK, Vernekar SN. Markers of renal function tests. North Am J Med Sci 2010:2:170-3 [PMID]
25. Ugbogu EA, Nwoku CD, Ude VC, Emmanuel O. Evaluating bioactive con- stituents and toxicological effects of aqueous extract of fermented Pentaclethra macro- phylla seeds in rats. Avicenna J Phytomed 2019:10 (1):101-13. [PMID]
26. Safhi MM. Nephroprotective effect of zingerone against CCl4-induced renal toxicity in Swiss albino mice: Molecular mechanism. Oxid Med Cell Longev 2018;2474831. [DOI:10.1155/2018/2474831] [PMID] []
27. Elsawy H, Badr GM, Sedky A, Abdallah BM, Alzahrani AM, Abdel-Moneim AM. Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats. Peer J 2019;7:e7011. [DOI:10.7717/peerj.7011] [PMID] []
28. Usunobun U, Inuumoru SC, Onyeaghor C, Lawuru D, Egbo H. Nephroprotective potential of Chromolaena odorata (L.) R.M. King & H.Rob. on Methotrexate-induced Kidney damage and Oxidative stress. J Res App Basic Med Sci 2022;8(3):118-127 [DOI:10.52547/rabms.8.3.118]
29. Usunobun U, Okolie PN. Effect of Annona muricata pre-treatment on liver synthetic ability, kidney function and hematological parameters in dimethylnitrosamine (DMN)-administered rats. Int J Med 2016;4(1):1-5. [DOI:10.14419/ijm.v4i1.5709]
30. Akinrinde AS, Oduwole O, Akinrinmade FJ, Bolaji-Alabi FB. Nephroprotective effect of methanol extract of Moringa oleifera leaves on acute kidney injury induced by ischemia-reperfusion in rats. Afr Health Sci 2020;20(3):1382-96 [DOI:10.4314/ahs.v20i3.44] [PMID] []
31. Sandhya VG, Rajamohan T. Comparative evaluation of the hypolipidemic effects of coconut water and lovastatin in rats fed fat-cholesterol enriched diet. Food Chem Toxicol 2008;46(12):3586-92. [DOI:10.1016/j.fct.2008.08.030] [PMID]
32. El-Hadary AE, Mohamed FRH. Hepatoprotective Effect of Cold- Pressed Syzygium aromaticum Oil against Carbon Tetrachloride (CCl4)-Induced Hepatotoxicity in Rats. Pharm Biol 2016;54:1364-72. [DOI:10.3109/13880209.2015.1078381] [PMID]
33. Dai N, Zou Y, Zhu L, Wang HF, Dai MG. Antioxidant Properties of Proanthocyanidins Attenuate Carbon Tetrachloride (CCl4)-induced Steatosis and Liver injury in rats via CYP2E1 Regulation. J Med Food 2014;17:663-9. [DOI:10.1089/jmf.2013.2834] [PMID] []
34. Maameri Z, Djerrou Z, Halmi S, Djaalab H, Riachi F, Hamdipacha Y. Evaluation of Hepatoprotective effect of Pistacia lentiscus L. Fatty Oil in rats intoxicated by Carbon Tetrachloride. Int J Pharmacogn Phytochem Res 2015:7:251-4. [DOI:10.3844/ojbsci.2015.36.41]
35. Usunobun U, Okolie PN, Eze GI. Effect of Vernonia amygdalina on Some Biochemical Indices in Dimethylnitrosamine (DMN)-induced Liver Injury in Rats. Int J Animal Biol 2015;1(4):99-105 [DOI:10.14419/ijpt.v4i1.5785]
36. Usunobun U, Okolie PN, Eze GI. Modulatory Effect of Ethanolic Leaf Extract of Annona muricata Pre-treatment on Liver Damage induced by Dimethylnitrosamine (DMN) in Rats. Br J Pharm Res 2015;8(3):1-9
https://doi.org/10.9734/BJPR/2015/19841 [DOI:10.9734/bjpr/2015/19841]
37. Che Idris CA, Wai Lin S, Abdull Razis AF. Hypocholesterolaemic and anti-atherogenic effects of palm-based oils (Novelin I and Novelin II) in cholesterol-fed rabbits. Inter J Environ Res Pub Health 2020;17(9):3226. [DOI:10.3390/ijerph17093226] [PMID] []
38. Emmanuel O, Okezie UM, Iweala EJ, Ugbogu EA. Pretreatment of red palm oil extracted from palm fruit (Elaeis guineensis) attenuates carbon tetrachloride induced toxicity in Wistar rats. Phytomedicine Plus 2021;1(4):100079. [DOI:10.1016/j.phyplu.2021.100079]
39. Al-shukor N, Raes K, Smagghe G, Camp JV. Flavonoids: evidence for inhibitory effects against obesity and their possible mechanisms of action. Flavonoids Antioxidants 2016;40:496-514. [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Contact Information
Address: Urmia University of Medical Sciences, Jahad Ave., Urmia, I.R. Iran. Post code: 57147-83734. P.O. Box. 1138
Email: ijrabms
gmail.com
rabmsumsu.ac.ir
Tel / Fax: +984431937332/+984432240642
