XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Aboutalebi E, Sakhinia E, Beilanokhi V, Dolatkhah H. Investigating the effect of Sclareol on IRE-1 and PERK genes the pathway of reticulandaplasmic system stress in gastric cancer cells MKN-45. Journal of Research in Applied and Basic Medical Sciences 2020; 6 (1) :44-32
URL: http://ijrabms.umsu.ac.ir/article-1-97-en.html
PhD in Clinical Biochemistry, Dept. of Clinical Biochemistry and Laboratories Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, East, Azarbaijan, Iran , dolatkhahh@gmail.com
Abstract:   (3431 Views)
Background & Aims:  Despite the decline in the prevalence of gastric cancer in recent years, it remains the fourth leading cause of death from cancer in the world. Common cancer treatments may reduce the size of the tumor, but it is transient and does not have a positive effect on the patient's survival and there is a possibility of recurrence of the disease. Strong induction of reticulom endoplasmic has been shown to increase the susceptibility to anti-cancer therapy. Regarding the importance of medicinal herbs in recent years and its low side effects after administration, compared with synthetic drugs, this study investigated the effects of salvia Sclareol purification from sage on the induction of reticulum endoplasmic system stress.
Materials and Methods: The MKN-45 cell line from the Pasteur Institute of Iran was purchased and cultured in a complete culture medium of RPMI-1640 with cetacean embryos. Cells cultured with 0, 20, 40, 60, 80 and 100 μm concentrations of Sclareol treatment for 5 hours. The rate of expression of IRE-1 and PERK genes by quantitative real time -PCR and the level of proteins IRE-1 and PERK by western blotting method was investigated.
Results: The rate of expression of IRE-1 in doses of 20, 40 and 60 μM Sclareol was significantly increased while decreasing in doses of 80 and 100 μM (p <0.0001). Also, the expression of PERK gene expression at doses of 20, 40 and 60 μM Sclareol was significantly increased, but no increase was observed in doses of 80 and 100 μM (p <0.0001). Also, the levels of IRE-1 and PERK proteins in doses of 20, 40 and 60 micromoles of Sclareol showed a high increase in doses of 80 and 100 μM.
Conclusion: From the results of this study, it seems that doses between 20 and 60 μmol of can be Sclareol helpful in increasing the amount of reticuloendoplasmic stress, but doses higher than 60 milimoles do not have like this effect.
Full-Text [PDF 1194 kb]   (1848 Downloads)    
Type of Study: orginal article | Subject: Special

1. Rafiemanesh H, Maleki F, Mohammadian-Hafshejani A, Salemi M, Salehiniya H. The trend in histological changes and the incidence of esophagus cancer in Iran (2003-2008). Int J Prev Med 2016;7:31. [DOI:10.4103/2008-7802.175990] [PMID] [PMCID]
2. Malekzadeh R, Mohammad H. Derakhshan, Malekzadeh Z. Gastric cancer in Iran: epidemiology and risk factors. Arch Iran Med 2009; 12 (6): 576-83.
3. Leone P, Vacca A, Dammacco F, Racanelli, V. Common variable immunodeficiency and gastric malignancies. Int J Molecular Sci 2018; 19(2):451. [DOI:10.3390/ijms19020451] [PMID] [PMCID]
4. Deng Z, Jiang H, Hu Y, Liu Y, Zhang L, Liu H, Li C. 3D Ordered Macroporous MoS2@ C Nanostructure for Flexible Li‐Ion Batteries. Adv Material 2017;29(10):1603020. [DOI:10.1002/adma.201603020] [PMID]
5. Bizzaro N, Antico A, Villalta D. Autoimmunity and Gastric Cancer. Int J Molecular Sci 2018;19(2):377. [DOI:10.3390/ijms19020377] [PMID] [PMCID]
6. Brown AGA, Vallenari A, Prusti T, De Bruijne JHJ, Babusiaux C, Bailer-Jones CAL, et al. Gaia Data Release 2-Summary of the contents and survey properties. Astronomy & astrophysics 2018;616:A1. [DOI:10.1051/0004-6361/201832964]
7. Diaz-Cano, Salvador J. Tumor heterogeneity: mechanisms and bases for a reliable application of molecular marker design. Int J Molecular Sci 2012; 13(2);: 1951-2011. [DOI:10.3390/ijms13021951] [PMID] [PMCID]
8. Genevieve H, Byler Sh, Heerboth S, Lapinska K, Longacre M, Snyder N, et al. Drug resistance in cancer: an overview. Cancers 2014; 6(3): 1769-92. [DOI:10.3390/cancers6031769] [PMID] [PMCID]
9. Yoboue ED, Sitia R, Simmen T. Redox crosstalk at endoplasmic reticulum (ER) membrane contact sites (MCS) uses toxic waste to deliver messages. Cell Death Dis 2018;9(3):331. [DOI:10.1038/s41419-017-0033-4] [PMID] [PMCID]
10. Ruggiano A, Foresti O, Carvalho P. Quality control: ER-associated degradation: protein quality control and beyond. J Cell Biol 2014;204(6):869-79. [DOI:10.1083/jcb.201312042] [PMID] [PMCID]
11. Prawira MR. The Analysis of the Turkish Hotile Policies aginst the Syrian Kurds 2015-2017. (Dissertation). Radboud University; 2017.
12. Davidson AAH, Dorner CS, Gradt DJ, LEE J, Manolis E, McDowell M, et al. Browsing interface for item counterparts having different scales and lengths [Internet]. US10026176B2, 2018 [cited 2019 Aug 3]. Available from: https://patents.google.com/patent/US10026176B2/en
13. Rahman S, Archana A, Jan AT, Minakshi R. Dissecting Endoplasmic Reticulum Unfolded Protein Response (UPRER) in Managing Clandestine Modus Operandi of Alzheimer's Disease. Front Aging Neurosci 2018;10:30. [DOI:10.3389/fnagi.2018.00030] [PMID] [PMCID]
14. Waters DW, Blokland KEC, Pathinayake PS, Burgess JK, Mutsaers SE, Prele CM, et al. Fibroblast senescence in the pathology of idiopathic pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 2018;315(2):L162-72. [DOI:10.1152/ajplung.00037.2018] [PMID] [PMCID]
15. Remondelli P, Renna M. The Endoplasmic Reticulum Unfolded Protein Response in Neurodegenerative Disorders and Its Potential Therapeutic Significance. Front Mol Neurosci 2017;10:187. [DOI:10.3389/fnmol.2017.00187] [PMID] [PMCID]
16. Remondelli P, Renna M. The Endoplasmic Reticulum Unfolded Protein Response in Neurodegenerative Disorders and Its Potential Therapeutic Significance. Front Mol Neurosci 2017;10:187. [DOI:10.3389/fnmol.2017.00187] [PMID] [PMCID]
17. Corazzari M, Gagliardi M, Fimia GM, Piacentini M. Endoplasmic Reticulum Stress, Unfolded Protein Response, and Cancer Cell Fate. Front Oncol 2017;7:78. [DOI:10.3389/fonc.2017.00078] [PMID] [PMCID]
18. Giampietri C, Petrungaro S, Conti S, Facchiano A, Filippini A, Ziparo E. Cancer Microenvironment and Endoplasmic Reticulum Stress Response. Mediators Inflamm 2015;2015:417281. [DOI:10.1155/2015/417281] [PMID] [PMCID]
19. Rozpedek W, Pytel D, Mucha B, Leszczynska H, Diehl JA, Majsterek I. The Role of the PERK/eIF2α/ATF4/CHOP Signaling Pathway in Tumor Progression During Endoplasmic Reticulum Stress. Curr Mol Med 2016;16(6):533-44. [DOI:10.2174/1566524016666160523143937] [PMID] [PMCID]
20. Noori M, Donald AE, Angelakopoulou A, Hingorani AD, Williams DJ. Clinical perspective. Circulation 2010;122(5):478-87. [DOI:10.1161/CIRCULATIONAHA.109.895458] [PMID]
21. Tamvakopoulos C, Dimas K, Sofianos ZD, Hatziantoniou S, Han Z, Liu Z-L, et al. Metabolism and anticancer activity of the curcumin analogue, dimethoxycurcumin. Clin Cancer Res 2007;13(4):1269-77. [DOI:10.1158/1078-0432.CCR-06-1839] [PMID]
22. Wang Y, Bryant SH, Cheng T, Wang J, Gindulyte A, Shoemaker BA, et al. PubChem BioAssay: 2017 update. Nucleic Acids Res 2017;45(D1):D955-63. [DOI:10.1093/nar/gkw1118] [PMID] [PMCID]
23. Han C, Zhang Z, Yu G, Huang F. Syntheses of a pillar[4]arene[1]quinone and a difunctionalized pillar[5]arene by partial oxidation. Chem Commun (Camb) 2012;48(79):9876-8. [DOI:10.1039/c2cc35498e] [PMID]
24. Signoretto E, Honisch S, Briglia M, Faggio C, Castagna M, Lang F. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem 2016;38(1):379-92. [DOI:10.1159/000438638] [PMID]
25. Cui G, Zhang F, Zhou X, Huang Y, Xuan X, Wang J. Acylamido-based anion-functionalized ionic liquids for efficient SO2 capture through multiple-site interactions. ACS Sustainable Chemistry & Engineering 2015;3(9):2264-70. [DOI:10.1021/acssuschemeng.5b00526]
26. Hatziantoniou S, Dimas K, Georgopoulos A, Sotiriadou N, Demetzos C. Cytotoxic and antitumor activity of liposome-incorporated sclareol against cancer cell lines and human colon cancer xenografts. Pharmacol Res 2006;53(1):80-7. [DOI:10.1016/j.phrs.2005.09.008] [PMID]
27. Jha S, Hassan M. Java implementation of policy-based bandwidth management. International Journal of Network Management 2003;13(4):249-58. [DOI:10.1002/nem.473]
28. Jha S, Hassan M. Java implementation of policy-based bandwidth management. International Journal of Network Management 2003;13(4):249-258. [DOI:10.1002/nem.473]
29. Wang X, Yue T-L, White RF, Barone FC, Feuerstein GZ. Transforming growth factor-β1 exhibits delayed gene expression following focal cerebral ischemia. Brain research bulletin 1995;36(6):607-9. [DOI:10.1016/0361-9230(94)00243-T]
30. Au S-K, Beck JL. Estimation of small failure probabilities in high dimensions by subset simulation. Probabilistic engineering mechanics 2001;16(4):263-77. [DOI:10.1016/S0266-8920(01)00019-4]
31. Peng Y, Lin W, Cai W, Arora R. Overexpression of a Panax ginseng tonoplast aquaporin alters salt tolerance, drought tolerance and cold acclimation ability in transgenic Arabidopsis plants. Planta 2007;226(3):729-40. [DOI:10.1007/s00425-007-0520-4] [PMID]
32. Sugasawara H, Yamamoto J. Computer-based teaching of word construction and reading in two students with developmental disabilities. Behavioral Interventions: Theory & Practice in Residential & Community-Based Clinical Programs 2007;22(4):263-277. [DOI:10.1002/bin.248]
33. Lacroix M, Leclercq G. Relevance of breast cancer cell lines as models for breast tumours: an update. Breast Cancer Res Treat 2004;83(3):249-89. [DOI:10.1023/B:BREA.0000014042.54925.cc] [PMID]
34. Shuda M, Kondoh N, Imazeki N, Tanaka K, Okada T, Mori K, et al. Activation of the ATF6, XBP1 and grp78 genes in human hepatocellular carcinoma: a possible involvement of the ER stress pathway in hepatocarcinogenesis. J Hepatol 2003;38(5):605-14. [DOI:10.1016/S0168-8278(03)00029-1]
35. Kulshreshtha R, Ferracin M, Wojcik SE, Garzon R, Alder H, Agosto-Perez FJ, et al. A microRNA signature of hypoxia. Molecular and cellular biology 2007;27(5):1859-67. [DOI:10.1128/MCB.01395-06] [PMID] [PMCID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Research in Applied and Basic Medical Sciences

Designed & Developed by : Yektaweb