Volume 6, Issue 4 (12-2020)
Journal of Research in Applied and Basic Medical Sciences 2020, 6(4): 199-206 |
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Mohammadpour M, Farjah G H. Protective Effect of Crocin on Ovarian Ischemia-Reperfusion Injury in Rats. Journal of Research in Applied and Basic Medical Sciences 2020; 6 (4) :199-206
URL: http://ijrabms.umsu.ac.ir/article-1-126-en.html
URL: http://ijrabms.umsu.ac.ir/article-1-126-en.html
Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran , Farjah.GH@umsu.ac.ir
Abstract: (2781 Views)
Background & Aims: Ischemia-reperfusion (I-R) injury of the ovary may lead to ovarian injury. In this study, we investigated the protective effect of the crocin on ovarian I-R injury.
Materials & Methods: Thirty-six male Sprague-Dawley rats were divided into 6 groups: sham surgery, ischemia, I-R, I-R+normal saline (NS), I-R+low dose crocin (20 mg/kg crocin), and I-R+ high dose crocin (80 mg/kg crocin). Neurological function, biochemical and histological evaluation was done 72 hours after ischemia.
Results: The plasma levels of malondialdehyde (MDA) and Total Antioxidative Capacity (TAC) in the ischemia, I-R, and I-R+ NS groups increased and decreased significantly compared to the crocin groups, respectively (p<0.01, p<0.05, respectively). Catalase activity in the high dose crocin group was higher than the ischemia, I-R, and I-R+ NS groups (p<0.01). The mean scores of edema, congestion, hemorrhage, and follicular degeneration were significantly lower than in the crocin groups than in the ischemia, I-R, and I-R+NS groups (p<0.05).
Conclusion: Findings suggest that crocin may protect ovary from ischemia-reperfusion injury.
Materials & Methods: Thirty-six male Sprague-Dawley rats were divided into 6 groups: sham surgery, ischemia, I-R, I-R+normal saline (NS), I-R+low dose crocin (20 mg/kg crocin), and I-R+ high dose crocin (80 mg/kg crocin). Neurological function, biochemical and histological evaluation was done 72 hours after ischemia.
Results: The plasma levels of malondialdehyde (MDA) and Total Antioxidative Capacity (TAC) in the ischemia, I-R, and I-R+ NS groups increased and decreased significantly compared to the crocin groups, respectively (p<0.01, p<0.05, respectively). Catalase activity in the high dose crocin group was higher than the ischemia, I-R, and I-R+ NS groups (p<0.01). The mean scores of edema, congestion, hemorrhage, and follicular degeneration were significantly lower than in the crocin groups than in the ischemia, I-R, and I-R+NS groups (p<0.05).
Conclusion: Findings suggest that crocin may protect ovary from ischemia-reperfusion injury.
Type of Study: orginal article |
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References
1. Gomes MM, Cavalcanti LS, Reis RL, Silva EJDCE, Dutra JB, de Melo-Leite AF. Twist and shout: magnetic resonance imaging findings in ovarian torsion. Radiol Bras 2019; 52(6):397-402. [DOI:10.1590/0100-3984.2018.0079] [PMID] [PMCID]
2. Feng JL, Zheng J, Lei T, Xu YJ, Pang H, Xie HN. Comparison of ovarian torsion between pregnant and non-pregnant women at reproductive ages: sonographic and pathological findings. Quant Imaging Med Surg 2020; 10(1):137-47. [DOI:10.21037/qims.2019.11.06] [PMID] [PMCID]
3. Mohammad Pour M, Farjah GH, Karimipour M, Pourheidar M, Khadam Ansari MH. Protective effect of lutein on spinal cord ischemia-reperfusion injury in rats. Iranian J Bascic Med sci 2019; 22(4):412-17.
4. Minutoli L, Puzzolo D, Rinaldi M, Irrera N, Marini H, Arcoraci V, et al. ROS-Mediated NLRP3 inflammasome activation in brain, heart, kidney, and testis Ischemia/Reperfusion Injury. Oxid Med Cell Longev 2016;2016:2183026. [DOI:10.1155/2016/2183026] [PMID] [PMCID]
5. Zhao H, Alam A, Soo AP, George AJT, Ma D. Ischemia-Reperfusion injury reduces long term renal graft survival: mechanism and beyond. EBioMedicine 2018;28:31-42. [DOI:10.1016/j.ebiom.2018.01.025] [PMID] [PMCID]
6. Tokgoz VY, Sipahi M, Keskin O, Guvendi GF, Takir S. Protective effects of vitamin D on ischemia-reperfusion injury of the ovary in a rat model. Iran J Basic Med Sci 2018;21(6):593-9. [PMID]
7. Balasubramaniam D, Duraisamy KY, Ezhilmani M. Laparoscopic detorsion and fertility preservation in twisted ischemic adnexa - A single - center prospective study. Gynecol Minim Invasive Ther 2020; 9 (1):24-8. [DOI:10.4103/GMIT.GMIT_20_19] [PMID] [PMCID]
8. Hortu I, Ilgen O, Sahin C, Akdemir A, Yigitturk G, Erbas O. Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental study. J Obstet Gynaecol 2020; 1-7. [DOI:10.1080/01443615.2019.1701639] [PMID]
9. Karaçor T, Dogan Z, Elibol E, Bulbul M, Nacar MC. Effects of iloprost on experimental ischemia and reperfusion injury in rat ovary. Biotec Histochem 2020; 95(3): 373-80. [DOI:10.1080/10520295.2019.1703219] [PMID]
10. Sakin O, Anğin AD, Akalin EE, Ciman MS, Basak K, Kaptanagasi AO. Comparison of the protective effects of sildenafil, vardenafil and tadalafil treatments in ischemia-reperfusion injury in rat ovary. Ginekol Pol 2019; 90 (2):513-9. [DOI:10.5603/GP.2019.0089] [PMID]
11. Farjah GH, Salehi S, Ansari MH, Pourheidar B. Protective effect of Crocus sativus L. (saffron) extract on spinal cord ischemia-reperfusion injury in rats. Iran J Basic Med Sci 2017; 20(3):334-37. [PMID]
12. Oncchiai T, soeda SH, Ohno s, tanaka h, shoyama y, shimeno h. crocin prevents the death of PC-12 cells through sphingomyelinase-ceramide signaling by increasing glutathione synthesis. Neurochem 2004; 44:321-30. [DOI:10.1016/S0197-0186(03)00174-8]
13. Liu W, Sun Y, Cheng Z, Guo Y, Liu P, Wen Y. Crocin exerts anti-inflammatory and anti-arthritic effects on type II collagen-induced arthritis in rats. Pharm Biol 2018;56(1):209-16. [DOI:10.1080/13880209.2018.1448874] [PMID] [PMCID]
14. Wang C, Cai X, Hu W, Li Z, Kong F, chen X, et al. Investigation of the neuroprotective effects of crocin via antioxidant activities in HT22 cells and in mice with Alzheimer's disease. Int J Mol Med 2019;43(2):956-66. [DOI:10.3892/ijmm.2018.4032]
15. Akbari G, Mard SA, Dianat M. Effect of crocin on cardiac antioxidants, and hemodynamic parameters after injuries induced by hepatic ischemia-reperfusion in rats. Iran J Basic Med Sci 2019; 22(3):277-81. [PMCID]
16. Mard SA, Azad SM, Ahangarpoor A. Protective effect of crocin on gastric mucosal lesions induced by ischemia-reperfusion injury in rats. Iran J Pharm Res 2016; 15 (Suppl):93-9. [PMID]
17. Zhang Y, Geng J, Hong Y, Jiao L, Li S, Sun R, et al. Orally administered crocin protects against cerebral ischemia/reperfusion injury through the metabolic transformation of vrocetin by gut microbiota. Front Pharmacol 2019; 10:440. [DOI:10.3389/fphar.2019.00440] [PMID] [PMCID]
18. Mozaffari S, Ramezany Yasuj S, Motaghinejad M, Motevalian M, Kheiri R. Crocin acting as a neuroprotective agent against methamphetamine-induced neurodegeneration via CREB-BDNF signaling pathway. Iran J Pharm Res 2019; 18(2):745-58. [PMCID]
19. Mokhber Maleki E, Eimani H, Bigdeli MR, Golkar Narenji A, Abedi R. Effects of Crocin Supplementation during In Vitro Maturation of Mouse Oocytes on Glutathione Synthesis and Cytoplasmic Maturation. Int J Fertil Steril 2016;10(1):53-61. [PMID]
20. Akdemir A, Erbas O, Gode F, Ergenoglu M, Yeniel O, Oltulu F. Protective effect of oxytocin on ovarian ischemia-reperfusion injury in rats. Peptides 2014; 55:126-30. [DOI:10.1016/j.peptides.2014.02.015] [PMID]
21. Oral A, Odabasoglu F, Halici Z, Keles ON, Unal B, Coskun AK, et al. Protective effects of montelukast on ischemia-reperfusion injury in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Fertil Steril 2011; 95(4):1360-6. [DOI:10.1016/j.fertnstert.2010.08.017] [PMID]
22. Yildirim N, Yigitturk G, Yildirim AGS, Akdemir A, Ilgen O, Yeniel O, et al. Octretide protects ovary against ischemia-reperfusion injury in rats: evaluation of histological and biochemical parameters. J Obstereics Gynaecology Res 2015; 41(10):1591-7. [DOI:10.1111/jog.12770] [PMID]
23. Karimipour M, Farjah GH, Molazadeh F, Ansari M, Pourheidar B. Protective effect of contralateral, ipsilateral, and bilateral remote ischemic preconditioning on spinal cord ischemia reperfusion injury in rats. Turk Neurosurg 2019; 29(6):933-9. [DOI:10.5137/1019-5149.JTN.26237-19.3] [PMID]
24. Tunc Sy, Agacayak E, Goruk NY, Icen MS, Turgut A, Alablik U, et al. Protective effects of honokiol on ischemia/reperfusion injury of rat ovary: an experimental study. Drug Des Devel Ther 2016; 10:1077-83. [DOI:10.2147/DDDT.S93768] [PMID] [PMCID]
25. Mard SA, Azad SM, Ahangarpoor A. Protective effect of crocin on gastric mucosal lesions induced by ischemia-reperfusion injury in rats. Iran J Pharm Res 2016; 15:93-9. [PMCID]
26. Sefidgar SM, Ahmadi-Hamedani M, Jebelli Javan A, Narenji Sani R, Javaheri Vayghan A. Effect of crocin on biochemical parameters, oxidative/antioxidative profiles, sperm characteristics and testicular histopathology in streptozotocin-induced diabetic rats. Avicenna J Phytomed 2019; 9(4):347-61. [PMID]
27. Wali AF, Rashid S, Rashid SM, Ansari MA, Khan MR, Haq N, et al. Naringenin regulates doxorubicin-unduced liver dysfunction: impact on oxidative stress and inflammation. Plants (Basel) 2020; 9(4): E550. [DOI:10.3390/plants9040550] [PMID] [PMCID]
28. Mohamadi Yarijani Z, Pourmotabbed A, Pourmotabbed T, Najafi H. Crocin has anti-inflammatory and protective effects in ischemia-reperfusion induced renal injuries. Iran J Basic Med Sci 2017; 20:753-9. [PMID]
29. Zhang P, Yue K, Liu X, Yan X, Yang Z, Duan J, et al. Endothelial notch activation promotes neutrophil transmigration via downregulating endomucin to aggravate hepatic ischemia/reperfusion injury. Sci China Life Sci 2020; 63(3):375-87. [DOI:10.1007/s11427-019-1596-4] [PMID]
30. Spears JR. Reperfusion microvascular ischemia after prolonged coronary occlus. Hypoxia (Auckl) 2019; 7:65-79. [DOI:10.2147/HP.S217955] [PMID] [PMCID]
31. Carbone F, Bonaventura A, Montecucco F. Neutrophil-related oxidants drive heart and brain remodeling after ischemia/reperfusion injury. Front Physiol 2020; 10:1587. [DOI:10.3389/fphys.2019.01587] [PMID] [PMCID]
32. Nie Z, Deng S, Zhang L, Chen S, Lu Q, Peng H. Crocin protects against dexamethasone-induced osteoblast apoptosis by inhibiting the ROS/Ca+- mediated mitochondrial pathway. Mol Med Rep 2019; 20(1):401-8. [DOI:10.3892/mmr.2019.10267]
33. Heidary S, Mehri S, Shariaty V, Hosseinzadeh H. Preventive effects of crocin on neuronal damages induced by D-galactose through AGEs and oxidative stress in human neuroblastoma cells (SH-SY5Y). J Pharmacopuncture 2018; 21(1):18-25. [DOI:10.3831/KPI.2018.21.003] [PMID] [PMCID]
34. Huang A, Jia L. Crocin enhances hypothermia therapy in hypoxic ischemia-induced brain injury in mice. Acta Neurol Belg 2019:1-8. [DOI:10.1007/s13760-019-01198-0] [PMID]
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